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Effect of Proteins Solubilized into AOT Reverse Micelles on their Back-extraction and Percolation Processes
http://hdl.handle.net/10458/5627
http://hdl.handle.net/10458/5627b3ea8750-df72-439b-ba4f-178422e2a5f6
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
本文 (375.5 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2020-06-21 | |||||
| タイトル | ||||||
| タイトル | Effect of Proteins Solubilized into AOT Reverse Micelles on their Back-extraction and Percolation Processes | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
久保井, 亮一
× 久保井, 亮一× 駒沢, 勲× 塩盛, 弘一郎× 河野, 恵宣× Kuboi, Ryoichi× Hong, Dong-pyo× Komasawa, Isao× Lee, Sung-sik |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The use of AOT reverse micellar systems （RVMS） for solubilization and back-extraction of proteins has been studied in relation to their percolation phenomena by using the injection method. Back-extraction of cytochrome c and hemoglobin into the aqueous phase was difficult, while catalase, C.C.lipase and BSA were more easily back-extracted depending on the pH. The significance of electrostatic interaction between the micelles and proteins is confirmed by comparing extraction results and their percolation processes. A sharp increase in electrical conductivity caused by the percolation phenomenon well demonstrates intermicellar interactions or stability of RVMS mediated with solubilized proteins. The value of β, defined as the variation of percolation threshold with the concentration of solubilized proteins, can be utilized to evaluate these interactions and to explain the results of back-extraction. The proteins which have positive or negative β values tend to have weak or strong interactions with micelles which results in a higher or lower degree of back-extraction, respectively. The β values are also affected by pH as well as by molecular weight, hydrophobicity and the types of proteins solubilized in the micelles. | |||||
| 書誌情報 |
Solvent Extraction Research and Development, Japan 巻 3, p. 223-230, 発行日 1996 |
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| 出版者 | ||||||
| 出版者 | Japan Association of Solvent Extraction | |||||
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| 出版者 | 日本溶媒抽出学会 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 13417215 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
