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Characterization of Bovine Heart Sulfotransferase Catalyzing the Sulfation of Tyrosine-Containing Peptides
http://hdl.handle.net/10458/1856
http://hdl.handle.net/10458/185658501c56-c66e-476c-abcf-67bd548f6983
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
JNSV43.pdf (1.0 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2009-02-24 | |||||
| タイトル | ||||||
| タイトル | Characterization of Bovine Heart Sulfotransferase Catalyzing the Sulfation of Tyrosine-Containing Peptides | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | Bovine heart, Sulfotransferase, Tyrosine sulfation | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Suiko, Masahito
× Suiko, Masahito× Fernando, P. H. Prasantha× 榊原, 陽一× Kudo, Hisao× 中村, 豊彦× Liu, Ming-Cheh |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Using [35S]PAPS as the sulfate donor, we have detected a sulfotransferase from bovine heart which catalyzes the sulfation of tyrosine-containing peptides. The enzyme displayed optimal activity at pH 5.75 and 35°C in a one-hour reaction. The addition of 10 mM Mn2+ or Co2+ to the reaction mixture increased the sulfotransferase activity by 3.4 and 3.5-fold, respectively. In contrast, the maximum increment stimulated by Mg2+ was only 1.75-fold at 15 mM concentration, and instead of exerting an enhancement effect, Ca2+ was found to be a potent inhibitor. The addition of 50 mM NaF to the reaction mixture resulted in an increase in sulfotransferase activity of 3.3-fold. The Km for 3'-phosphoadenosine 5'-phosphosulfate （PAPS） was determined to be 2μM at a constant 0.5 mM Boc-Glu-Asp-Tyr-Val. Among the 10 peptides tested as substrates, Boc-Glu-Asp-Tyr-Val and Boc-Asp-Asp-Tyr-Val provided the highest activities. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Journal of nutritional science and vitaminology en : J. nutr. sci. vitaminol 巻 43, p. 485-490, 発行日 1997-08 |
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| 出版者 | ||||||
| 出版者 | 日本ビタミン学会 | |||||
| 言語 | ja | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 03014800 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00703822 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
