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'晩白柚'と四倍体の正逆交雑から得られた種子の重さと倍数性との関係
http://hdl.handle.net/10458/1848
http://hdl.handle.net/10458/1848d80827e3-11d6-4223-a782-31ac35424872
名前 / ファイル | ライセンス | アクション |
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HR2-4.pdf (1.5 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-02-24 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | '晩白柚'と四倍体の正逆交雑から得られた種子の重さと倍数性との関係 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Relationship between Weight of Seeds and Ploidy Level of the Seed- Derived Plantlets Obtained by the Reciprocal Crosses between 'Banpeiyu' pummelo and Tetraploid Citrus Species | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
言語 | ja | |||||
主題Scheme | Other | |||||
キーワード | 倍数性育種, 晩白柚, フローサイトメトリー, カンキツ, 三倍体 | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
著者 |
八幡, 昌紀
× 八幡, 昌紀× 岡, 信孝× 國武, 久登× 山口, 清二× 小松, 春喜× Yahata, Masaki× Oka, Nobutaka× Yamaguchi, Seiji× Komatsu, Haruki |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 晩白柚'を用いた三倍体育種を効率的に進めるために,二倍体と四倍体間での正逆交雑から得られた種子の重さとその倍数性との関係を調査した.'晩白柚'に四倍体ユズまたは四倍体ナツダイダイを交雑したところ,完全種子出現率が対照区(放任受粉果)と比較してそれぞれ48.0%と8.4%まで減少した.それらの実生の倍数性を調査した結果,三倍体と四倍体が確認され,前者の組合せからは六倍体が1個体得られた,'晩白柚'×四倍体ユズでは三倍体の発生率が高く(88.0%),'晩白柚'×四倍体ナツダイダイでは低かった(30.2%).二倍体×四倍体の両組合せから得られた三倍体と四倍体の平均種子重は,三倍体が四倍体より有意に軽いことが明らかとなった.さらに,これらの組合せから得られた不完全種子を培養した結果,得られた実生はすべて三倍体であった.一方,四倍体ユズおよび四倍体ナツダイダイに'晩白柚'を交雑した結果,放任受粉果と比較して,それぞれの完全種子出現率には大きな差異は観察されなかったものの,種子重は有意に減少した.三倍体は,珠心胚由来と考えられる四倍体に混じって,それぞれの組合せで約50%の頻度で得られた.三倍体が発生した種子とそれ以外の種子の重さには有意な差異は観察されなかった. \nRelationship between weight of seeds and ploidy level of the seed-derived plantlets obtained from reciprocal crosses between diploid 'Banpeiyu' pummelo and two tetraploid varieties were examined to efficiently select triploid progenies for further breeding of the pummelo. Percentages of fully developed seeds obtained from the cross of 'Banpeiyu' pummelo with 4X Yuzu or 4X Natsudaidai were decreased by 48.0% and 8.4% as compared with the control, respectively. Seedlings obtained from these two crosses included triploids, tetraploids and hexaploid. 'Banpeiyu' pummelo as seed parent yielded a high percentage of triploids (88.0%) when crossed with 4X Yuzu, but low percentage (30.2%) when crossed with 4X Natsudaidai. In both crosses, the average weight of triploid seeds was significantly lighter than that of tetraploid seeds. Furthermore, all seedlings derived from undeveloped seeds were triploids. Percentages of fully developed seeds obtained from 4X Yuzu or 4X Natsudaidai X 'Banpeiyu' pummelo were not different from those of seeds from open pollinated fruits, but weights of these seeds were extremely decreased compared to seeds from open pollinated fruits. Approximately 50% of in vitro cultured seedlings obtained from both crosses were triploid, while the others were tetraploids, which might be derived from nucellar embryos of seed parents. There was no difference in the average weights of seeds between have seeds yielding triploid seedlings and those yielding tetraploids that might have originated from nucellar tissue. |
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言語 | ja | |||||
書誌情報 |
ja : 園芸学研究 en : Horticultural research (Japan) 巻 2, 号 4, p. 247-252, 発行日 2003-12-15 |
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出版者 | ||||||
言語 | ja | |||||
出版者 | 園芸学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13472658 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11608561 | |||||
権利 | ||||||
言語 | ja | |||||
権利情報 | 園芸学会 | |||||
著者版フラグ | ||||||
出版タイプ | VoR |