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Regulatory mechanisms of fowl sperm motility: Possible role of endogenous myosin light chain kinase-like protein
http://hdl.handle.net/10458/714
http://hdl.handle.net/10458/7147dea4dfa-a0cd-40a3-a9f2-f7911880b11d
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ashizawa54.pdf (1.7 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2007-10-10 | |||||
| タイトル | ||||||
| タイトル | Regulatory mechanisms of fowl sperm motility: Possible role of endogenous myosin light chain kinase-like protein | |||||
| 言語 | en | |||||
| タイトル | ||||||
| タイトル | Myosin light chain kinase and fowl sperm motility | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | sperm, spermatozoa, Motility, myosin light chain kinase, protein phosphorylation, fowl | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
芦沢, 幸二
× 芦沢, 幸二× Wishart, G.J.× Nishinakama, K.× Sakamoto, T.× Tsuzuki, Y. |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The motility of both intact and demembranated fowl spermatozoa was vigorous at 30°C, but decreased markedly following the addition of 1-（5-chloronaphthalene-1-sulfonyl）-1H-hexahydro-1,4-diazepine hydrochloride （ML-9）, a specific inhibitor of myosin light chain kinase （MLCK）. Furthermore, the presence of a MLCK substrate peptide also inhibited the motility of demembranated spermatozoa at 30°C. In contrast, the addition of N-[2-（methylamino）ethyl]-5-isoquinolinesulfonamide dihydrochloride （H-8） or N-（2-guanidinoethyl）-5-isoquinolinesulfonamide hydrochloride （HA1004）, specific inhibitors of cAMP-dependent protein kinase, did not appreciably affect the motility of either intact or demembranated spermatozoa. Cyclic AMP-dependent protein kinase substrate peptides were also ineffective for the inhibition of motility of demembranated spermatozoa at 30°C. Immunoblotting of sperm extract using an antibody to MLCK revealed two major cross-reacting proteins of 130 kDa and 61-64 kDa, which corresponded to the molecular mass of MLCK. In addition, immunogold particles which reacted with the anti-MLCK antibody were observed around/or on the axoneme at the ultrastructural level. These results suggest that the phosphorylation of axonemal protein（s） by MLCK, or a MLCK-like protein, rather than by cAMP-dependent protein kinase, may be involved in the maintenance of fowl sperm motility at 30°C. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Disclaimer. This is not the definitive version of record of this article. This manuscript has been accepted for publication in Reproduction, but the version presented here has not yet been copy edited, formatted or proofed. Consequently, neither Reproduction, the Society for Reproduction and Fertility nor its publisher, BioScientifica Ltd, accepts responsibility for any errors or omissions it may contain. The definitive version is now freely available at [insert DOI link]. 1995 Society for Reproduction and Fertility. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Journal of reproduction and fertility 巻 104, 号 1, p. 141-148, 発行日 1995 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00224251 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00705952 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
