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  1. 農学部
  2. 学術雑誌掲載論文 (農学部)

Regulatory mechanisms of fowl sperm motility: Possible role of endogenous myosin light chain kinase-like protein

http://hdl.handle.net/10458/714
http://hdl.handle.net/10458/714
7dea4dfa-a0cd-40a3-a9f2-f7911880b11d
名前 / ファイル ライセンス アクション
ashizawa54.pdf ashizawa54.pdf (1.7 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2007-10-10
タイトル
タイトル Regulatory mechanisms of fowl sperm motility: Possible role of endogenous myosin light chain kinase-like protein
言語 en
タイトル
タイトル Myosin light chain kinase and fowl sperm motility
言語 en
言語
言語 eng
キーワード
言語 en
主題Scheme Other
キーワード sperm, spermatozoa, Motility, myosin light chain kinase, protein phosphorylation, fowl
資源タイプ
資源タイプ journal article
著者 芦沢, 幸二

× 芦沢, 幸二

WEKO 932

ja 芦沢, 幸二

en Ashizawa, Koji

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Wishart, G.J.

× Wishart, G.J.

WEKO 1536

en Wishart, G.J.

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Nishinakama, K.

× Nishinakama, K.

WEKO 2320

en Nishinakama, K.

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Sakamoto, T.

× Sakamoto, T.

WEKO 2321

en Sakamoto, T.

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Tsuzuki, Y.

× Tsuzuki, Y.

WEKO 2322

en Tsuzuki, Y.

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抄録
内容記述タイプ Abstract
内容記述 The motility of both intact and demembranated fowl spermatozoa was vigorous at 30°C, but decreased markedly following the addition of 1-(5-chloronaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine hydrochloride (ML-9), a specific inhibitor of myosin light chain kinase (MLCK). Furthermore, the presence of a MLCK substrate peptide also inhibited the motility of demembranated spermatozoa at 30°C. In contrast, the addition of N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide dihydrochloride (H-8) or N-(2-guanidinoethyl)-5-isoquinolinesulfonamide hydrochloride (HA1004), specific inhibitors of cAMP-dependent protein kinase, did not appreciably affect the motility of either intact or demembranated spermatozoa. Cyclic AMP-dependent protein kinase substrate peptides were also ineffective for the inhibition of motility of demembranated spermatozoa at 30°C. Immunoblotting of sperm extract using an antibody to MLCK revealed two major cross-reacting proteins of 130 kDa and 61-64 kDa, which corresponded to the molecular mass of MLCK. In addition, immunogold particles which reacted with the anti-MLCK antibody were observed around/or on the axoneme at the ultrastructural level. These results suggest that the phosphorylation of axonemal protein(s) by MLCK, or a MLCK-like protein, rather than by cAMP-dependent protein kinase, may be involved in the maintenance of fowl sperm motility at 30°C.
言語 en
内容記述
内容記述タイプ Other
内容記述 Disclaimer. This is not the definitive version of record of this article. This manuscript has been accepted for publication in Reproduction, but the version presented here has not yet been copy edited, formatted or proofed. Consequently, neither Reproduction, the Society for Reproduction and Fertility nor its publisher, BioScientifica Ltd, accepts responsibility for any errors or omissions it may contain. The definitive version is now freely available at [insert DOI link]. 1995 Society for Reproduction and Fertility.
言語 en
書誌情報 en : Journal of reproduction and fertility

巻 104, 号 1, p. 141-148, 発行日 1995
ISSN
収録物識別子タイプ ISSN
収録物識別子 00224251
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00705952
著者版フラグ
出版タイプ AM
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