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Stimulation of motility and respiration of intact fowl spermatozoa by calyculin A, a specific inhibitor of protein phosphatase-1 and -2A, via a Ca2+-dependent mechanism
http://hdl.handle.net/10458/715
http://hdl.handle.net/10458/715599a470a-f0dd-48bc-9aef-a7fc3d94a5de
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ashizawa55.pdf (1.3 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2007-10-10 | |||||
| タイトル | ||||||
| タイトル | Stimulation of motility and respiration of intact fowl spermatozoa by calyculin A, a specific inhibitor of protein phosphatase-1 and -2A, via a Ca2+-dependent mechanism | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | ja | |||||
| 主題Scheme | Other | |||||
| キーワード | 精子 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | sperm, spermatozoa, protein phosphorylation, protein dephosphorylation, motility, oxygen consumption, ATP concentration, calcium | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| その他（別言語等）のタイトル | ||||||
| その他のタイトル | Calyculin A and fowl sperm functions | |||||
| 言語 | en | |||||
| 著者 |
芦沢, 幸二
× 芦沢, 幸二× Magome, A.× 續木, 靖浩 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Intact fowl spermatozoa without addition of calyculin A, a specific inhibitor of protein phosphatase-1 (PP1) and -2A (PP2A), became almost immotile at 40°C. In contrast, the presence of 10-1000 nmol calyculin A 1-1 permitted activation of sperm motility in a dose-dependent manner. Calyculin A also stimulated the rate of sperm oxygen consumption, and induced a concomitant decrease in ATP concentrations, suggesting a coupling of ATP hydrolysis to the rate of oxidative phosphorylation. However, the motility and oxygen consumption of spermatozoa loaded with an intracellular Ca2+ chelator, 1,2-bis (2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid, tetraacetoxymethyl ester (BAPTA/AM), were not stimulated by calyculin A alone, but only after the subsequent addition of 2 mmol CaCl2 l-1. These results suggest that inhibition of the activities of endogenous PP1 and/or PP2A activities may stimulate the motility and metabolic activity of fowl spermatozoa at 40°C via a mechanism which requires intracellular free Ca2+. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Disclaimer. This is not the definitive version of record of this article. This manuscript has been accepted for publication in Reproduction, but the version presented here has not yet been copy edited, formatted or proofed. Consequently, neither Reproduction, the Society for Reproduction and Fertility nor its publisher, BioScientifica Ltd, accepts responsibility for any errors or omissions it may contain. The definitive version is now freely available at . 1995 Society for Reproduction and Fertility. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Journal of reproduction and fertility 巻 105, 号 1, p. 109-114, 発行日 1995 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00224251 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00705952 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
