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Inhibition of Flagellar Motility of Demembranated Fowl Spermatozoa by Protease Substrates
http://hdl.handle.net/10458/728
http://hdl.handle.net/10458/728a8f9949c-53e5-4e9e-955a-e0db083bbb71
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ashizawa64.pdf (1.2 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-10-10 | |||||
| タイトル | ||||||
| タイトル | Inhibition of Flagellar Motility of Demembranated Fowl Spermatozoa by Protease Substrates | |||||
| 言語 | en | |||||
| タイトル | ||||||
| タイトル | Protease and fowl sperm motility | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | ja | |||||
| 主題Scheme | Other | |||||
| キーワード | 精子 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | sperm, dephosphorylation, fowl, fragellar motility, phosphorylation, protease, temperature-dependent, spermatozoa, substrate | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
芦沢, 幸二
× 芦沢, 幸二× Hashimoto, Kei× Suzuki, Yoshie× Tsuzuki, Ysuhiro |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We investigated the effects of various protease substrates on the motility of demembranated fowl spermatozoa. In the presence of ATP, the motility of demembranated spermatozoa was vigorous at 30°C, but decreased markedly following the addition of protease substrates, such as Nα-carbobenzoxy-l-lys-thiobenzyl ester （BLT）, N-benzoyl-phe-val-arg p-nitroanilide or Nα-benzoyl-d,l-arg p-nitroanilide （BAPNA） in a dose-dependent manner, within the range 0-1 mM. The subsequent addition of 100 ng/ml trypsin released the inhibitory effect of protease substrates within 10 sec. Phosphorylation or dephosphorylation of several proteins of demembranated spermatozoa was observed following the addition of protease substrates, however, no consistent patterns of protein phosphorylation or dephosphorylation were associated with the inhibition of motility. These results suggest that endogenous protease activity is instrumental in the maintenance of fowl sperm motility and that the site of action of this protease is in the axoneme and/or accessory cytoskeletal components. This enzyme may not act directly on the phosphorylation of sperm proteins involved in the regulation of motility. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 巻 121, 号 1, p. 77-83, 発行日 1998 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 10956433 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11183804 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
