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Maintenance of fowl sperm motility in vitro is prolonged by a low molecular weight factor derived from cultured chick embryo cells
http://hdl.handle.net/10458/703
http://hdl.handle.net/10458/703a4a89920-cbea-49ed-989b-d7ef543b1ac3
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ashizawa38.pdf (958.6 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-10-10 | |||||
| タイトル | ||||||
| タイトル | Maintenance of fowl sperm motility in vitro is prolonged by a low molecular weight factor derived from cultured chick embryo cells | |||||
| 言語 | en | |||||
| タイトル | ||||||
| タイトル | Maintenance of motility of fowl spermatozoa in vitro is prolonged by a low molecular weight factor derived from cultured chick embryo cells|cultured cells and survival of fowl sperm | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | ja | |||||
| 主題Scheme | Other | |||||
| キーワード | 精子 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | sperm, spermatozoa, motility, embryonic cells, conditioned medium, fowl | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
芦沢, 幸二
× 芦沢, 幸二× Katayama, S. |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Gel filtration of a conditioned medium （CM）composed of the supernatant fluid removed from a 5-day culture of skeletal muscle cells from 9-day-old chick embryos with Bio-Gel P-2 revealed one peak of motility-prolonging activity （Mol. Wt., about 300 daltons）, which was not present in fresh medium （FM）. Spermatozoa incubated in this fraction of the CM maintained their motility for at least 36h at 37°C. Both the formation of lipid peroxide and the leakage of lactic dehydrogenase of spermatozoa incubated in the CM fraction were lower than those incubated in the corresponding FM. Initial rate of oxygen consumption of the spermatozoa incubated in the conditioned medium fraction increased compared with that of the FM fraction. These results suggest that a low molecular weight factor（s） supplied by cultured cells effectively prolongs the motility of fowl spermatozoa, and that the effect could result from inhibition of the structural damage to the sperm membrane. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Disclaimer. This is not the definitive version of record of this article. This manuscript has been accepted for publication in Reproduction, but the version presented here has not yet been copy edited, formatted or proofed. Consequently, neither Reproduction, the Society for Reproduction and Fertility nor its publisher, BioScientifica Ltd, accepts responsibility for any errors or omissions it may contain. The definitive version is now freely available at insert DOI link]. 1992 Society for Reproduction and Fertility. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Journal of reproduction and fertility 巻 95, 号 3, p. 685-691, 発行日 1992 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00224251 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00705952 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
