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  1. 農学部
  2. 学術雑誌掲載論文 (農学部)

Maintenance of fowl sperm motility in vitro is prolonged by a low molecular weight factor derived from cultured chick embryo cells

http://hdl.handle.net/10458/703
http://hdl.handle.net/10458/703
a4a89920-cbea-49ed-989b-d7ef543b1ac3
名前 / ファイル ライセンス アクション
ashizawa38.pdf ashizawa38.pdf (958.6 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2007-10-10
タイトル
タイトル Maintenance of fowl sperm motility in vitro is prolonged by a low molecular weight factor derived from cultured chick embryo cells
言語 en
タイトル
タイトル Maintenance of motility of fowl spermatozoa in vitro is prolonged by a low molecular weight factor derived from cultured chick embryo cells|cultured cells and survival of fowl sperm
言語 en
言語
言語 eng
キーワード
言語 ja
主題Scheme Other
キーワード 精子
キーワード
言語 en
主題Scheme Other
キーワード sperm, spermatozoa, motility, embryonic cells, conditioned medium, fowl
資源タイプ
資源タイプ journal article
著者 芦沢, 幸二

× 芦沢, 幸二

WEKO 932

ja 芦沢, 幸二

en Ashizawa, Koji

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Katayama, S.

× Katayama, S.

WEKO 1721

en Katayama, S.

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抄録
内容記述タイプ Abstract
内容記述 Gel filtration of a conditioned medium (CM)composed of the supernatant fluid removed from a 5-day culture of skeletal muscle cells from 9-day-old chick embryos with Bio-Gel P-2 revealed one peak of motility-prolonging activity (Mol. Wt., about 300 daltons), which was not present in fresh medium (FM). Spermatozoa incubated in this fraction of the CM maintained their motility for at least 36h at 37°C. Both the formation of lipid peroxide and the leakage of lactic dehydrogenase of spermatozoa incubated in the CM fraction were lower than those incubated in the corresponding FM. Initial rate of oxygen consumption of the spermatozoa incubated in the conditioned medium fraction increased compared with that of the FM fraction. These results suggest that a low molecular weight factor(s) supplied by cultured cells effectively prolongs the motility of fowl spermatozoa, and that the effect could result from inhibition of the structural damage to the sperm membrane.
言語 en
内容記述
内容記述タイプ Other
内容記述 Disclaimer. This is not the definitive version of record of this article. This manuscript has been accepted for publication in Reproduction, but the version presented here has not yet been copy edited, formatted or proofed. Consequently, neither Reproduction, the Society for Reproduction and Fertility nor its publisher, BioScientifica Ltd, accepts responsibility for any errors or omissions it may contain. The definitive version is now freely available at insert DOI link]. 1992 Society for Reproduction and Fertility.
言語 en
書誌情報 en : Journal of reproduction and fertility

巻 95, 号 3, p. 685-691, 発行日 1992
ISSN
収録物識別子タイプ ISSN
収録物識別子 00224251
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00705952
著者版フラグ
出版タイプ AM
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芦沢, 幸二, Katayama, S., 1992, Maintenance of motility of fowl spermatozoa in vitro is prolonged by a low molecular weight factor derived from cultured chick embryo cells|cultured cells and survival of fowl sperm: 685–691 p.

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