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Aspergillus nigerの生産する細胞外エンド型イヌリナ-ゼ(P-III)の固定化と性質
http://hdl.handle.net/10458/4546
http://hdl.handle.net/10458/454691bea707-afb8-4276-a0a7-4cf462e0bee9
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
oota_2.pdf (2.0 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-07-05 | |||||
| タイトル | ||||||
| タイトル | Aspergillus nigerの生産する細胞外エンド型イヌリナ-ゼ(P-III)の固定化と性質 | |||||
| 言語 | ja | |||||
| タイトル | ||||||
| タイトル | Properties and Immobilization of Extracellular Endoinulinase(P-3) from Aspergillus niger | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Inulinase, Aspergillus niger, Immobilized enzyme, Inulin | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
中村, 豊彦
× 中村, 豊彦× 藤田, 平× 太田, 一良× Fujita, Taira |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Our previous study demonstrated that the extracellular inulinase from a strain, Aspergillus niger 12 is a new endo-inulinase which is specific only to inulin but not to sucrose and raffinose etc. In this study, the endo-inulinase purified to electrophoretically homogeneous was immobilized onto amino-cellulofine according to carbodiimide coupling method. The activity yield of the immobilized enzyme was 35%. The characteristics of the immobilized enzyme were compared with those of the native enzyme. The optimun pH was around 5.0 for the both enzyme and the optimum temperatures were 45°C for the native and 40°C for the immobilized enzyme. These results indicate that the immobilization resulted in the lowering of optimum temperature by 5°C. In addition, pH stability of the enzyme became broader and temperature stability was raised by approximately 10°C by the immobilization. Then, the effects of various reagents on the enzyme activity were examined. An activation by Mn^2+, and strong inhibitions by Hg^2+, Ag^+ and NBS were found for both enzymes. |
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| 言語 | en | |||||
| 書誌情報 |
ja : 宮崎大学農学部研究報告 en : Bulletin of the Faculty of Agriculture, Miyazaki University 巻 43, 号 2, p. 103-109, 発行日 1997-02 |
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| 出版者 | ||||||
| 出版者 | 宮崎大学農学部 | |||||
| 言語 | ja | |||||
| 出版者 | ||||||
| 出版者 | Miyazaki University | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 05446066 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00236503 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
