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REGULATORY SYSTEM OF TEMPERATURE-DEPENDENT FLAGELLAR MOTILITY IS RETAINED AFTER FREEZING AND THAWING OF DEMEMBRANATED FOWL SPERMATOZOA
http://hdl.handle.net/10458/720
http://hdl.handle.net/10458/720bc8cf221-ef72-4311-8aff-f6b7e98f1062
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ashizawa57.pdf (1.1 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-10-10 | |||||
| タイトル | ||||||
| タイトル | REGULATORY SYSTEM OF TEMPERATURE-DEPENDENT FLAGELLAR MOTILITY IS RETAINED AFTER FREEZING AND THAWING OF DEMEMBRANATED FOWL SPERMATOZOA | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | ja | |||||
| 主題Scheme | Other | |||||
| キーワード | 精子 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | sperm, spermatozoa, motility, freezing, temperature, fowl | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
芦沢, 幸二
× 芦沢, 幸二× Okabe, H.× Tsuzuki, Y. |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In the presence of adenosine 5'-triphosphate （ATP）, the motility of demembranated fowl spermatozoa was vigorous at 30°C, both before and after rapid freezing at -70°C or -196°C without cryoprotectants. The rate of ATP consumption of the demembranated spermatozoa treated by freezing and thawing was, at approximately 200 nmol ATP hydrolysis/109 spermatozoa/min, also similar to that of the untreated spermatozoa. At 40°C, neither frozen- thawed spermatozoa nor control spermatozoa were motile, but motility could be restored by decreasing the temperature to 30°C or by the addition of inhibitors of protein phosphatases. These observations suggest that the substance（s） involved in the temperature-dependent immobilization of fowl spermatozoa are closely associated with the axoneme, and that physical damage to the demembranated spermatozoa, such as freezing and thawing, is not enough to remove the substance（s）. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Theriogenology : an international journal of animal reproduction 巻 46, 号 8, p. 1413-1424, 発行日 1996 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0093691X | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00862972 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
