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Mesurement of intracellular free Ca2+ concentration of fowl spermatozoa using fura-2 and the relationship between intracellular Ca2+ and the motility or respiration of spermatozoa
http://hdl.handle.net/10458/706
http://hdl.handle.net/10458/7067f91b6b2-20a7-440e-a8aa-3af47781f089
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ashizawa41.pdf (1.5 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2007-10-10 | |||||
| タイトル | ||||||
| タイトル | Mesurement of intracellular free Ca2+ concentration of fowl spermatozoa using fura-2 and the relationship between intracellular Ca2+ and the motility or respiration of spermatozoa | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | ja | |||||
| 主題Scheme | Other | |||||
| キーワード | 精子 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | sperm, spermatozoa, calcium, fura-2, motility, oxygen uptake, fowl | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| その他（別言語等）のタイトル | ||||||
| その他のタイトル | Intracellular free Ca2+ concentration in fowl spermatozoa and its relationship to motility and respiration in spermatozoa|Intracellular Ca2+ and motility of fowl sperm | |||||
| 言語 | en | |||||
| 著者 |
芦沢, 幸二
× 芦沢, 幸二× Hashiguchi, A.× 續木, 靖浩 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The ability of fowl spermatozoa to accumulate and de-esterify the intracellular fluorescent Ca2+ indicator fura-2 was established. The cytosolic Ca2+ concentrations, measured by this technique, did not change after the addition of 1 mmol EGTA l-1. Subsequently, addition of the calcium ionophore A23187 caused a reduction in cytosolic Ca2+ concentrations, presumably by efflux of Ca2+ from the spermatozoa. Intracellular free Ca2+ concentrations were then significantly increased by the addition of 1 mmol CaCl2 l-1. The motility of demembranated spermatozoa gradually decreased after the addition of EGTA alone or EGTA with A23187. However, the motility was instantly restored by the addition of CaCl2 in the presence of both EGTA and A23187. Unlike demembranated spermatozoa, intact spermatozoa maintained their motility, even after the addition of EGTA. However, their motility was reduced by the addition of A23187 in the presence of EGTA. The addition of A23187 also reduced the rate of oxygen consumption, but not the ATP concentrations in intact spermatozoa. These results demonstrate that the motility and respiration of fowl spermatozoa are strongly influenced by their intracellular Ca2+ concentrations. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Disclaimer. This is not the definitive version of record of this article This manuscript has been accepted for publication in Reproduction, but the version presented here has not yet been copy edited, formatted or proofed. Consequently, neither Reproduction, the Society for Reproduction and Fertility nor its publisher, BioScientifica Ltd, accepts responsibility for any errors or omissions it may contain. The definitive version is now freely available at [insert DOI link]. 1992 Society for Reproduction and Fertility. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Journal of reproduction and fertility 巻 96, 号 1, p. 395-405, 発行日 1992 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00224251 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00705952 | |||||
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| 出版タイプ | AM | |||||
