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Channel induction by palytoxin in yeast cells expressing Na+,K+-ATPase or its chimera with sarco/endoplasmic reticulum Ca2+-ATPase
http://hdl.handle.net/10458/1131
http://hdl.handle.net/10458/11311f7cd6cf-9337-4c94-ae67-7e3131da3b8f
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
FEBS543_108_2003.pdf (117.5 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2008-01-30 | |||||
| タイトル | ||||||
| タイトル | Channel induction by palytoxin in yeast cells expressing Na+,K+-ATPase or its chimera with sarco/endoplasmic reticulum Ca2+-ATPase | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | Palytoxin, Na+,K+-ATPase, Channel, Catalytic domain, Yeast, Chimera | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
伊藤, 勝昭
× 伊藤, 勝昭× 伊藤, 勝昭× Toyoda, Isao× Higashiyama, Masato× Uemura, Daisuke× Sato, Masa H.× Yoshimura, Shige H.× Ishii, Toshiaki× Takeyasu, Kunio |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Palytoxin （PTX） induces a cation channel through interaction with Na+,K+-ATPase. It is unclear how this action relates to the enzyme catalytic activity. We examined whether the action of PTX depends on the catalytic domain specific for Na+,K+-ATPase. Wild-type Na+,K+-ATPase α-subunit （NNN） or its chimera （NCN）, in which the catalytic domain was replaced with that of SERCA, was co-expressed with β-subunit in the yeast Saccharomyces cerevisiae. PTX （0.1-100 nM） increased K+ efflux in NNN- or NCN-transfected cells to a similar degree but not in non-transfected cells. When ouabain-resistant NNN and NCN were expressed, PTX also increased K+ efflux. Ouabain inhibited the effect of PTX in NNN- or NCN-cells but not in ouabain-resistant cells. These data suggest that the channel-forming action of PTX does not depend on the catalytic domain species. |
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| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | <a href="http://www.febsletters.com/" target="_blank">Journal HP</a> | |||||
| 言語 | en | |||||
| 書誌情報 |
en : FEBS letters : for the rapid publication of short reports in biochemistry, biophysics and molecular biology 巻 543, 号 1-3, p. 108-112, 発行日 2003-05-22 |
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| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00145793 | |||||
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| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00642943 | |||||
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| 出版タイプ | AM | |||||
