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アイテム
Expression of fibroblast activation protein-α in human deep vein thrombosis
http://hdl.handle.net/10458/0002000874
http://hdl.handle.net/10458/000200087436a954b5-f9e1-4ecb-a63b-69036f28110e
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
出版社版
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||
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| 公開日 | 2024-12-25 | |||||||||||||||||||
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| タイトル | Expression of fibroblast activation protein-α in human deep vein thrombosis | |||||||||||||||||||
| 言語 | en | |||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| キーワード | α2-antiplasmin | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| キーワード | Deep vein thrombosis | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| キーワード | Fibroblast | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| キーワード | Fibroblast activation protein-α | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| キーワード | Organization | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| キーワード | Podoplanin | |||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||
| アクセス権 | ||||||||||||||||||||
| 著者 |
Oguri, Nobuyuki
× Oguri, Nobuyuki
× 魏, 峻洸× 中村, 恵理子× 古小路, 英二× 後藤, 洋規× 前川, 和也× Tsuji, Atsushi B.
× Nishii, Ryuichi
× 阿萬, 紫× 盛口, 清香
WEKO
9238
× 榮, 建文× 東, 美菜子× 山下, 篤 |
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| 抄録 | ||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||
| 内容記述 | Background Fibroblast activation protein-α (FAP), a type-II transmembrane serine protease, is associated with wound healing, cancer-associated fibroblasts, and chronic fibrosing diseases. However, its expression in deep vein thrombosis (DVT) remains unclear. Therefore, this study investigated FAP expression and localization in DVT. Methods We performed pathological analyses of the aspirated thrombi of patients with DVT (n = 14), classifying thrombotic areas in terms of fresh, cellular lysis, and organizing reaction components. The organizing reaction included endothelialization and fibroblastic reaction. We immunohistochemically examined FAP-expressed areas and cells, and finally analyzed FAP expression in cultured dermal fibroblasts. Results All the aspirated thrombi showed a heterogeneous mixture of at least two of the three thrombotic areas. Specifically, 83 % of aspirated thrombi showed fresh and organizing reaction components. Immunohistochemical expression of FAP was restricted to the organizing area. Double immunofluorescence staining showed that FAP in the thrombi was mainly expressed in vimentin-positive or α-smooth muscle actin-positive fibroblasts. Some CD163-positive macrophages expressed FAP. FAP mRNA and protein levels were higher in fibroblasts with low-proliferative activity cultured under 0.1 % fetal bovine serum (FBS) than that under 10 % FBS. Fibroblasts cultured in 10 % FBS showed a significant decrease in FAP mRNA levels following supplementation with hemin, but not with thrombin. Conclusions The heterogeneous composition of venous thrombi suggests a multistep thrombus formation process in human DVT. Further, fibroblasts or myofibroblasts may express FAP during the organizing process. FAP expression may be higher in fibroblasts with low proliferative activity. |
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| 言語 | en | |||||||||||||||||||
| 書誌情報 |
en : Thrombosis Research 巻 241, p. 109075-109075 |
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| 出版者 | ||||||||||||||||||||
| 出版者 | Elsevier BV | |||||||||||||||||||
| 言語 | en | |||||||||||||||||||
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| 収録物識別子タイプ | PISSN | |||||||||||||||||||
| 収録物識別子 | 0049-3848 | |||||||||||||||||||
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| 関連タイプ | isVersionOf | |||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||
| 関連識別子 | https://doi.org/10.1016/j.thromres.2024.109075 | |||||||||||||||||||
| 権利 | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 権利情報 | © 2024 The Authors. Published by Elsevier Ltd. | |||||||||||||||||||
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| 出版タイプ | VoR | |||||||||||||||||||
