The crucial role of SETDB1 in structural and functional transformation of epithelial cells during regeneration after intestinal ischemia reperfusion injury

池ノ上, 実; イケノウエ, マコト; Ikenoue, Makoto; Choijookhuu, Narantsog; チヨウジヨウフ, ナランツオツク; チヨウジヨウフ, ナランツオツク; Yano, Koichiro; Fidya; 高橋, 伸育; タカハシ, ノブヤス; Takahashi, Nobuyasu; 石塚, 匠; イシヅカ, タクミ; Ishizuka, Takumi; 白水, 慎一郎; シロウズ, シンイチロウ; Shirouzu, Shinichiro; 山隈, 優; ヤマグマ, ユウ; Yamaguma, Yu; 甲斐, 健吾; カイ, ケンゴ; Kai, Kengo; 樋口, 和宏; ヒグチ, カズヒロ; Higuchi, Kazuhiro; 澤口, 朗; サワグチ, アキラ; Sawaguchi, Akira; 七島, 篤志; ナナシマ, アツシ; Nanashima, Atsushi; 菱川, 善隆; ヒシカワ, ヨシタカ; Hishikawa, Yoshitaka

doi:https://doi.org/10.1007/s00418-023-02263-9

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The crucial role of SETDB1 in structural and functional transformation of epithelial cells during regeneration after intestinal ischemia reperfusion injury

http://hdl.handle.net/10458/0002000739

Item type

学術雑誌論文 / Journal Article(1)

公開日

2024-09-06

タイトル

The crucial role of SETDB1 in structural and functional transformation of epithelial cells during regeneration after intestinal ischemia reperfusion injury

言語

eng

キーワード

言語

キーワード

SETDB1

キーワード

言語

キーワード

Jejunum remodeling

キーワード

言語

キーワード

Ischemia reperfusion injury

キーワード

言語

キーワード

Sinefungin

資源タイプ

journal article

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metadata only access

著者

池ノ上, 実

WEKO 34693
e-Rad_Researcher 40612370

ja	池ノ上, 実宮崎大学
ja-Kana	イケノウエ, マコト
en	Ikenoue, Makoto University of Miyazaki

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チヨウジヨウフ, ナランツオツク

WEKO 26967
e-Rad_Researcher 90640962

en	Choijookhuu, Narantsog
ja	チヨウジヨウフ, ナランツオツク
ja-Kana	チヨウジヨウフ, ナランツオツク

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Yano, Koichiro
Fidya
高橋, 伸育

WEKO 34847
e-Rad_Researcher 20404436

ja	高橋, 伸育宮崎大学
ja-Kana	タカハシ, ノブヤス
en	Takahashi, Nobuyasu University of Miyazaki

Search repository

石塚, 匠

WEKO 30147
e-Rad_Researcher 50700085

ja	石塚, 匠宮崎大学
ja-Kana	イシヅカ, タクミ
en	Ishizuka, Takumi University of Miyazaki

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白水, 慎一郎

WEKO 34832

ja	白水, 慎一郎宮崎大学
ja-Kana	シロウズ, シンイチロウ
en	Shirouzu, Shinichiro University of Miyazaki

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山隈, 優

WEKO 34808

ja	山隈, 優宮崎大学
ja-Kana	ヤマグマ, ユウ
en	Yamaguma, Yu University of Miyazaki

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甲斐, 健吾

WEKO 33883
e-Rad_Researcher 90851585

ja	甲斐, 健吾宮崎大学
ja-Kana	カイ, ケンゴ
en	Kai, Kengo University of Miyazaki

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樋口, 和宏

WEKO 35379
e-Rad_Researcher 90979361

ja	樋口, 和宏宮崎大学
ja-Kana	ヒグチ, カズヒロ
en	Higuchi, Kazuhiro University of Miyazaki

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澤口, 朗

WEKO 21832
e-Rad_Researcher 30336292

ja	澤口, 朗宮崎大学
ja-Kana	サワグチ, アキラ
en	Sawaguchi, Akira University of Miyazaki

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七島, 篤志

WEKO 33886
e-Rad_Researcher 60380838

ja	七島, 篤志宮崎大学
ja-Kana	ナナシマ, アツシ
en	Nanashima, Atsushi University of Miyazaki

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菱川, 善隆

WEKO 34189
e-Rad_Researcher 60304276

ja	菱川, 善隆宮崎大学
ja-Kana	ヒシカワ, ヨシタカ
en	Hishikawa, Yoshitaka University of Miyazaki

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抄録

内容記述タイプ

Abstract

内容記述

Su (var) 3–9, enhancer of seste, trithorax (SET)-domain bifurcated histone lysine methyltransferase (SETDB1) plays a crucial role in maintaining intestinal stem cell homeostasis; however, its physiological function in epithelial injury is largely unknown. In this study, we investigated the role of SETDB1 in epithelial regeneration using an intestinal ischemia/reperfusion injury (IRI) mouse model. Jejunum tissues were sampled after 75 min of ischemia followed by 3, 24, and 48 h of reperfusion. Morphological evaluations were performed using light microscopy and electron microscopy, and the involvement of SETDB1 in epithelial remodeling was investigated by immunohistochemistry. Expression of SETDB1 was increased following 24 h of reperfusion and localized in not only the crypt bottom but also in the transit amplifying zone and part of the villi. Changes in cell lineage, repression of cell adhesion molecule expression, and decreased histone H3 methylation status were detected in the crypts at the same time. Electron microscopy also revealed aberrant alignment of crypt nuclei and fusion of adjacent villi. Furthermore, increased SETDB1 expression and epithelial remodeling were confirmed with loss of stem cells, suggesting SETDB1 affects epithelial cell plasticity. In addition, crypt elongation and increased numbers of Ki-67 positive cells indicated active cell proliferation after IRI; however, the expression of PCNA was decreased compared to sham mouse jejunum. These morphological changes and the aberrant expression of proliferation markers were prevented by sinefungin, a histone methyltransferase inhibitor. In summary, SETDB1 plays a crucial role in changes in the epithelial structure after IRI-induced stem cell loss.

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Citation:
Ikenoue, M., Choijookhuu, N., Yano, K. et al. The crucial role of SETDB1 in structural and functional transformation of epithelial cells during regeneration after intestinal ischemia reperfusion injury. Histochem Cell Biol 161, 325–336 (2024). https://doi.org/10.1007/s00418-023-02263-9

言語

書誌情報

en : Histochemistry and Cell Biology

巻 161, 号 4, p. 325-336, 発行日 2024-01-13

出版者

Springer Science and Business Media LLC

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ISSN

収録物識別子タイプ

PISSN

収録物識別子

0948-6143

ISSN

収録物識別子タイプ

EISSN

収録物識別子

1432-119X

DOI

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2024-09-06 00:30:21.711956

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The crucial role of SETDB1 in structural and functional transformation of epithelial cells during regeneration after intestinal ischemia reperfusion injury

× 池ノ上, 実

× チヨウジヨウフ, ナランツオツク

× Yano, Koichiro

× Fidya

× 高橋, 伸育

× 石塚, 匠

× 白水, 慎一郎

× 山隈, 優

× 甲斐, 健吾

× 樋口, 和宏

× 澤口, 朗

× 七島, 篤志

× 菱川, 善隆

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