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Development of an efficient screening system to identify novel bone metabolism-related genes using the exchangeable gene trap mutagenesis mouse models
http://hdl.handle.net/10458/0002000486
http://hdl.handle.net/10458/0002000486601e5c3b-ddc5-4f8e-9f50-8d0ada675471
| 名前 / ファイル | ライセンス | アクション |
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article (2 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||||||||||||||||||
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| 公開日 | 2024-02-05 | |||||||||||||||||||||||||||||||||||
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| タイトル | Development of an efficient screening system to identify novel bone metabolism-related genes using the exchangeable gene trap mutagenesis mouse models | |||||||||||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||||||||||||||||
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| 資源タイプ | journal article | |||||||||||||||||||||||||||||||||||
| 著者 |
黒木, 修司
× 黒木, 修司
WEKO
28474
× 関本, 朝久× 舩元, 太郎
WEKO
34184
× 大田, 智美
WEKO
8099
× 中村, 志保子× 永井, 琢哉
WEKO
28480
× Nakahara, Mai× Yoshinobu, Kumiko× Araki, Kimi× Araki, Masatake× 帖佐, 悦男
WEKO
7830
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| 内容記述タイプ | Abstract | |||||||||||||||||||||||||||||||||||
| 内容記述 | Despite numerous genetic studies on bone metabolism, understanding of the specific mechanisms is lacking. We developed an efficient screening system to identify novel genes involved in bone metabolism using mutant mouse strains registered with the Exchangeable Gene Trap Clones (EGTC) database. From 1278 trap clones in the EGTC database, 52 candidate lines were selected in the first screening, determined based on “EST profile”, “X-gal”, “Related article”, and “Novel gene”. For the second screening, bone morphometric analysis, biomechanical strength analysis, bone X-gal staining, etc. were performed on candidate lines. Forty-two male trap lines (80.8%) showed abnormalities with either bone morphometric analysis or biomechanical strength analysis. In the screening process, X-gal staining was significantly efficient (P = 0.0057). As examples, Lbr and Nedd4 trap lines selected using the screening system showed significant bone decrease and fragility, suggesting a relationship with osteoblast differentiation. This screening system using EGTC mouse lines is extremely efficient for identifying novel genes involved in bone metabolism. The gene trap lines identified as abnormal using this screening approach are highly likely to trap important genes for bone metabolism. These selected trap mice will be valuable for use as novel bio-resources in bone research. | |||||||||||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||||||||||
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| 内容記述タイプ | Other | |||||||||||||||||||||||||||||||||||
| 内容記述 | Citation: Kurogi S, Sekimoto T, Funamoto T, Ota T, Nakamura S, Nagai T, Nakahara M, Yoshinobu K, Araki K, Araki M, Chosa E. Development of an efficient screening system to identify novel bone metabolism-related genes using the exchangeable gene trap mutagenesis mouse models. Sci Rep. 2017 Jan 20;7:40692. doi: 10.1038/srep40692. PMID: 28106071; PMCID: PMC5247747. |
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| 言語 | en | |||||||||||||||||||||||||||||||||||
| 書誌情報 |
en : Scientific Reports 巻 7, 号 1, p. 40692, 発行日 2017-01-20 |
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| 出版者 | SpringerNature | |||||||||||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||||||||||
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| 関連タイプ | isVersionOf | |||||||||||||||||||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1038/srep40692 | |||||||||||||||||||||||||||||||||||
| 権利 | ||||||||||||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||||||||||
| 権利情報 | © The Author(s) 2017 | |||||||||||||||||||||||||||||||||||
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| 出版タイプ | VoR | |||||||||||||||||||||||||||||||||||
