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Characterization of human telomere RNA G-quadruplex structures in vitro and in living cells using 19F NMR spectroscopy
http://hdl.handle.net/10458/0002000441
http://hdl.handle.net/10458/0002000441aecda199-8889-4981-b0b5-58a89cfdaa83
| 名前 / ファイル | ライセンス | アクション |
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article (3 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
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| 公開日 | 2024-01-22 | |||||||||
| タイトル | ||||||||||
| タイトル | Characterization of human telomere RNA G-quadruplex structures in vitro and in living cells using 19F NMR spectroscopy | |||||||||
| 言語 | en | |||||||||
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| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ | journal article | |||||||||
| 著者 |
鮑, 宏亮
× 鮑, 宏亮× 石塚, 匠× Sakamoto, Takashi
× Fujimoto, Kenzo
× 上地, 珠代× 剣持, 直哉× 徐, 岩 |
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| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Human telomeric RNA has been identified as a key component of the telomere machinery. Recently, the growing evidence suggests that the telomeric RNA forms G-quadruplex structures to play an important role in telomere protection and regulation. In the present studies, we developed a 19F NMR spectroscopy method to investigate the telomeric RNA G-quadruplex structures in vitro and in living cells. We demonstrated that the simplicity and sensitivity of 19F NMR approach can be used to directly observe the dimeric and two-subunits stacked G-quadruplexes in vitro and in living cells and quantitatively characterize the thermodynamic properties of the G-quadruplexes. By employing the 19F NMR in living cell experiment, we confirmed for the first time that the higher-order G-quadruplex exists in cells. We further demonstrated that telomere RNA G-quadruplexes are converted to the higher-order G-quadruplex under molecular crowding condition, a cell-like environment. We also show that the higher-order G-quadruplex has high thermal stability in crowded solutions. The finding provides new insight into the structural behavior of telomere RNA G-quadruplex in living cells. These results open new avenues for the investigation of G-quadruplex structures in vitro and in living cells. | |||||||||
| 言語 | en | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | Citation: Bao HL, Ishizuka T, Sakamoto T, Fujimoto K, Uechi T, Kenmochi N, Xu Y. Characterization of human telomere RNA G-quadruplex structures in vitro and in living cells using 19F NMR spectroscopy. Nucleic Acids Res. 2017 May 19;45(9):5501-5511. doi: 10.1093/nar/gkx109. PMID: 28180296; PMCID: PMC5435947. |
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| 言語 | en | |||||||||
| 書誌情報 |
en : Nucleic Acids Research 巻 45, 号 9, p. 5501-5511, 発行日 2017-02-19 |
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| 出版者 | ||||||||||
| 出版者 | Oxford University Press | |||||||||
| 言語 | en | |||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 0305-1048 | |||||||||
| DOI | ||||||||||
| 関連タイプ | isVersionOf | |||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | https://doi.org/10.1093/nar/gkx109 | |||||||||
| 権利 | ||||||||||
| 言語 | en | |||||||||
| 権利情報 | © 2024 Oxford University Press © The Author(s) 2017 | |||||||||
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| 出版タイプ | VoR | |||||||||
