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Development of LAMP assays for the molecular detection of taeniid infection in canine in Tibetan rural area
http://hdl.handle.net/10458/0002000418
http://hdl.handle.net/10458/000200041828c148fb-8458-4656-8005-bbd5b9f99ae9
| 名前 / ファイル | ライセンス | アクション |
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article (782 KB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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| 公開日 | 2023-12-27 | |||||||
| タイトル | ||||||||
| タイトル | Development of LAMP assays for the molecular detection of taeniid infection in canine in Tibetan rural area | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| キーワード | canine | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| キーワード | China | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| キーワード | diagnosis | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| キーワード | taeniid cestodes | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| キーワード | zoonosis | |||||||
| 資源タイプ | ||||||||
| 資源タイプ | journal article | |||||||
| 著者 |
Feng, Kai
× Feng, Kai× Li, Wei× 郭, 志宏× Duo, Hong× Fu, Yong× Shen, Xiuying× Tie, Cheng× E, Rijie× Xiao, Changqin× Luo, Yanhong× Qi, Guo× Ni, Ma× Ma, Qingmei× Yamazaki, Wataru× 吉田, 彩子× 堀井, 洋一郎× Yagi, Kinpei
× 野中, 成晃 |
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | For field-identification of taeniid cestodes in canine animals in Tibetan area, loop-mediated isothermal amplification (LAMP) assays for Echinococcus multilocularis, E. shiquicus, Taenia hydatigena, T. multiceps, T. pisiformis and T. crassiceps were developed and evaluated along with the reported assay for E. granulosus. The LAMP assays showed specific reaction with their corresponding target species DNA with the detection limit of 1 to 10 pg. Moreover, the assays for E. granulosus, E. multilocularis, T. hydatigena and T. multiceps could detect DNA extracted from 3 or more eggs of their corresponding target species. Then, the LAMP assays were applied on samples containing 3 to 35 taeniid eggs obtained from 61 field-collected canine feces in Qinghai, and the result was compared with a reported multiplex PCR and sequence analysis. The LAMP assays and the PCR detected single species DNA of E. granulosus, E. shiquicus, T. hydatigena and T. multiceps in 5, 2, 44 and 2 samples, respectively. In the rest 8 samples, DNA of both E. granulosus and T. hydatigena were detected by the PCR but the LAMP assays detected those DNAs in 2 samples and only T. hydatigena DNA in 6 samples. It was assumed that less than 3 E. granulosus eggs were mixed in the samples although the samples contained 21 to 27 eggs in total. In conclusion, the LAMP assays were less sensitive than the multiplex PCR, but would have adequate sensitivity for field use in Tibetan area. | |||||||
| 言語 | en | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | Citation: Feng K, Li W, Guo Z, Duo H, Fu Y, Shen X, Tie C, E R, Xiao C, Luo Y, Qi G, Ni M, Ma Q, Yamazaki W, Yoshida A, Horii Y, Yagi K, Nonaka N. Development of LAMP assays for the molecular detection of taeniid infection in canine in Tibetan rural area. J Vet Med Sci. 2017 Dec 22;79(12):1986-1993. doi: 10.1292/jvms.17-0430. Epub 2017 Oct 23. PMID: 29057765; PMCID: PMC5745177. |
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| 言語 | en | |||||||
| 書誌情報 |
en : Journal of Veterinary Medical Science 巻 79, 号 12, p. 1986-1993, 発行日 2017-10-23 |
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| 出版者 | ||||||||
| 出版者 | Japanese Society of Veterinary Science | |||||||
| 言語 | en | |||||||
| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 0916-7250 | |||||||
| DOI | ||||||||
| 関連タイプ | isVersionOf | |||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | https://doi.org/10.1292/jvms.17-0430 | |||||||
| 権利 | ||||||||
| 言語 | en | |||||||
| 権利情報 | © 2017 by the Japanese Society of Veterinary Science | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
