| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2023-12-26 |
| タイトル |
|
|
タイトル |
DNA hypermethylation enhanced telomerase reverse transcriptase expression in human-induced pluripotent stem cells |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
キーワード |
DNA methylation |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
キーワード |
Epigenetics |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
キーワード |
Human TERT gene |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
キーワード |
Human iPSCs |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
キーワード |
Lamin B1 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
キーワード |
Reprogramming |
| 資源タイプ |
|
|
資源タイプ |
journal article |
| 著者 |
髙澤, 建
新井, 良和
Yamazaki-Inoue Mayu
Toyoda Masashi
Akutsu Hidenori
Umezawa Akihiro
西野, 光一郎
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
During reprogramming into human induced pluripotent stem cells (iPSCs), several stem cell marker genes are induced, such as OCT-4, NANOG, SALL4, and TERT. OCT-4, NANOG, and SALL4 gene expression can be regulated by DNA methylation. Their promoters become hypomethylated in iPSCs during reprogramming, leading to their induced expression. However, epigenetic regulation of the TERT gene remains unclear. In this study, we focused on epigenetic regulation of the human TERT gene and identified a differentially methylated region (DMR) at a distal region in the TERT promoter between human iPSCs and their parental somatic cells. Interestingly, the TERT-DMR was highly methylated in iPSCs, but low-level methylation was observed in their parental somatic cells. Region-specific, methylated-promoter assays showed that the methylated TERT-DMR up-regulated the promoter activity in iPSCs. In addition, Lamin B1 accumulated at the TERT-DMR in iPSCs, but not in their parent somatic cells. These results suggested that the TERT transcription was enhanced by DNA methylation at the TERT-DMR via binding to nuclear lamina during reprogramming. Our findings shed light on a new functional aspect of DNA methylation in gene expression. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
citation:
Takasawa K, Arai Y, Yamazaki-Inoue M, Toyoda M, Akutsu H, Umezawa A, Nishino K. DNA hypermethylation enhanced telomerase reverse transcriptase expression in human-induced pluripotent stem cells. Hum Cell. 2018 Jan;31(1):78-86. doi: 10.1007/s13577-017-0190-x. Epub 2017 Nov 4. PMID: 29103143. |
|
言語 |
en |
| 書誌情報 |
en : Human Cell
巻 31,
号 1,
p. 78-86,
発行日 2018-01-31
|
| 出版者 |
|
|
出版者 |
Springer Nature |
|
言語 |
en |
| DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1007/s13577-017-0190-x |
| 権利 |
|
|
言語 |
en |
|
権利情報 |
© 2023 Springer Nature |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
article (1.1 MB)
