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アイテム
Arsenic trioxide induces ROS activity and DNA damage, leading to G0/G1 extension in skin fibroblasts through the ATM-ATR-associated Chk pathway
http://hdl.handle.net/10458/0002000408
http://hdl.handle.net/10458/0002000408908db8ac-b582-4204-bb3d-611aacf9b195
| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||||
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| 公開日 | 2023-12-22 | |||||||||||||||||||||
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| タイトル | Arsenic trioxide induces ROS activity and DNA damage, leading to G0/G1 extension in skin fibroblasts through the ATM-ATR-associated Chk pathway | |||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| キーワード | Arsenic trioxide | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| キーワード | DNA damage | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| キーワード | G0/G1 | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| キーワード | Cell cycle arrest | |||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||
| アクセス権 | ||||||||||||||||||||||
| 著者 |
Chayapong, Jutapon
× Chayapong, Jutapon× マドゥエスタ, ハリーシャクマール
WEKO
7810
× Madhyastha, Radha× クイーン, インタン ヌラーマ× 中島, 融一× チヨウジヨウフ, ナランツオツク
WEKO
26967
× 菱川, 善隆× 丸山, 眞杉 |
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| 抄録 | ||||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||||
| 内容記述 | Arsenic (As) toxicity is a global health problem, affecting millions of people. Exposure to arsenic, mostly via drinking water, has been associated with cancer of skin, lungs, and blood, in addition to several kinds of skin lesions. The present study focused on the effect of arsenic trioxide (As2O3) on normal skin fibroblast cells. Specifically, the effect of As2O3 on ROS generation and oxidative stress was investigated. Proteins involved in the DNA damage signaling pathway and cell cycle were also studied. As2O3 induced the generation of intracellular ROS. Immunohistochemistry analysis revealed a dose-dependent increase in the number of 8-OHdG-positive cells, an indication of oxidative stress. Cell cycle analysis by flow cytometry demonstrated that As2O3 caused a significant percentage of cells to accumulate in the G0/G1 phase with a concomitant reduction in the S phase. Increases in the activated forms of DNA damage signaling proteins, ATM and ATR, and their effector molecules, Chk2 and p53, were also observed. In addition, expression of oncogene p21 was also increased. The study shows that exposure of normal skin fibroblast cells to As2O3 could lead to cell cycle arrest through ATM/ATR and DNA damage signaling pathways. In conclusion, we report here that arsenic trioxide increases cellular oxidative stress leading to shift in cell cycle and leads to DNA damage through ATM/ATR and the CHK-dependent signaling pathway. | |||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 内容記述 | ||||||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||||||
| 内容記述 | Citation: Chayapong J, Madhyastha H, Madhyastha R, Nurrahmah QI, Nakajima Y, Choijookhuu N, Hishikawa Y, Maruyama M. Arsenic trioxide induces ROS activity and DNA damage, leading to G0/G1 extension in skin fibroblasts through the ATM-ATR-associated Chk pathway. Environ Sci Pollut Res Int. 2017 Feb;24(6):5316-5325. doi: 10.1007/s11356-016-8215-7. Epub 2016 Dec 24. PMID: 28013460. |
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| 言語 | en | |||||||||||||||||||||
| 書誌情報 |
en : Environmental Science and Pollution Research 巻 24, 号 6, p. 5316-5325, 発行日 2017 |
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| 出版者 | Springer | |||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||
| 収録物識別子 | 0944-1344 | |||||||||||||||||||||
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| 関連タイプ | isVersionOf | |||||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1007/s11356-016-8215-7 | |||||||||||||||||||||
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| 出版タイプ | NA | |||||||||||||||||||||
