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Analysis of DNA Methylation in Bowel Lavage Fluid for Detection of Colorectal Cancer
http://hdl.handle.net/10458/0002000403
http://hdl.handle.net/10458/0002000403983cb81c-8269-4c80-bc64-a85f5334454b
| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||
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| 公開日 | 2023-12-21 | |||||||||||||||
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| タイトル | Analysis of DNA Methylation in Bowel Lavage Fluid for Detection of Colorectal Cancer | |||||||||||||||
| 言語 | en | |||||||||||||||
| 言語 | ||||||||||||||||
| 言語 | eng | |||||||||||||||
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| 資源タイプ | journal article | |||||||||||||||
| アクセス権 | ||||||||||||||||
| 著者 |
原田, 拓
× 原田, 拓× Yamamoto, Eiichiro× Yamano, Hiro-o× Nojima, Masanori× Maruyama, Reo
× Kumegawa, Kohei
× Ashida, Masami
× Yoshikawa, Kenjiro× Kimura, Tomoaki× Harada, Eiji× Takagi, Ryo× Tanaka, Yoshihito× Aoki, Hironori
× Nishizono, Masayo× Nakaoka, Michiko× Tsuyada, Akihiro× Niinuma, Takeshi× Kai, Masahiro
× 下田, 和哉× Shinomura, Yasuhisa× Sugai, Tamotsu× Imai, Kohzoh× Suzuki, Hiromu |
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| 抄録 | ||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||
| 内容記述 | Aberrant DNA methylation could potentially serve as a biomarker for colorectal neoplasms. In this study, we assessed the feasibility of using DNA methylation detected in bowel lavage fluid (BLF) for colorectal cancer screening. A total of 508 BLF specimens were collected from patients with colorectal cancer (n = 56), advanced adenoma (n = 53), minor polyp (n = 209), and healthy individuals (n = 190) undergoing colonoscopy. Methylation of 15 genes (miR-1-1, miR-9-1, miR-9-3, miR-34b/c, miR-124-1, miR-124-2, miR-124-3, miR-137, SFRP1, SFRP2, APC, DKK2, WIF1, LOC386758, and ZNF582) was then analyzed in MethyLight assays, after which receiver operating characteristic (ROC) curves were analyzed to assess the diagnostic performance of BLF methylation. Through analyzing BLF specimens in a training set (n = 345), we selected the three genes showing the greatest sensitivity for colorectal cancer detection (miR-124-3, 71.8%; LOC386758, 79.5%; and SFRP1, 74.4%). A scoring system based on the methylation of those three genes (M-score) achieved 82% sensitivity and 79% specificity, and the area under the ROC curve (AUC) was 0.834. The strong performance of this system was then validated in an independent test set (n = 153; AUC = 0.808). No significant correlation was found between M-score and the clinicopathologic features of the colorectal cancers. Our results demonstrate that DNA methylation in BLF specimens may be a useful biomarker for the detection of colorectal cancer. | |||||||||||||||
| 言語 | en | |||||||||||||||
| 内容記述 | ||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||
| 内容記述 | Citation: Harada T, Yamamoto E, Yamano HO, Nojima M, Maruyama R, Kumegawa K, Ashida M, Yoshikawa K, Kimura T, Harada E, Takagi R, Tanaka Y, Aoki H, Nishizono M, Nakaoka M, Tsuyada A, Niinuma T, Kai M, Shimoda K, Shinomura Y, Sugai T, Imai K, Suzuki H. Analysis of DNA methylation in bowel lavage fluid for detection of colorectal cancer. Cancer Prev Res (Phila). 2014 Oct;7(10):1002-10. doi: 10.1158/1940-6207.CAPR-14-0162. Epub 2014 Aug 19. PMID: 25139296. |
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| 言語 | en | |||||||||||||||
| 書誌情報 |
en : Cancer Prevention Research 巻 7, 号 10, p. 1002-1010, 発行日 2014-10-01 |
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| 出版者 | ||||||||||||||||
| 出版者 | American Association for Cancer Research | |||||||||||||||
| 言語 | en | |||||||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||||||
| 収録物識別子 | 1940-6207 | |||||||||||||||
| DOI | ||||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||
| 関連識別子 | https://doi.org/10.1158/1940-6207.CAPR-14-0162 | |||||||||||||||
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原田, 拓, Yamamoto, Eiichiro, Yamano, Hiro-o, Nojima, Masanori, Maruyama, Reo, Kumegawa, Kohei, Ashida, Masami, Yoshikawa, Kenjiro, Kimura, Tomoaki, Harada, Eiji, Takagi, Ryo, Tanaka, Yoshihito, Aoki, Hironori, Nishizono, Masayo, Nakaoka, Michiko, Tsuyada, Akihiro, Niinuma, Takeshi, Kai, Masahiro, 下田, 和哉, Shinomura, Yasuhisa, Sugai, Tamotsu, Imai, Kohzoh, Suzuki, Hiromu, 2014, Analysis of DNA Methylation in Bowel Lavage Fluid for Detection of Colorectal Cancer: American Association for Cancer Research, 1002–1010 p.
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