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In vitro synergistic activities of cefazolin and nisin A against mastitis pathogens
http://hdl.handle.net/10458/0002000397
http://hdl.handle.net/10458/0002000397e7e762ff-168a-4e8b-83c0-f21ab2da0dba
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
article (1.1 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2023-12-20 | |||||
| タイトル | ||||||
| タイトル | In vitro synergistic activities of cefazolin and nisin A against mastitis pathogens | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | cattle | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | cephalosporin | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | mastitis | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | nisin | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | synergism | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
北崎, 宏平
× 北崎, 宏平× Koga, Shoko× Nagatoshi, Kohei× Kuwano, Koichi× Zendo, Takeshi× Nakayama, Jiro× Sonomoto, Kenji× 阿野, 仁志× 片本, 宏 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | First-generation cephalosporins such as cefazolin (CEZ) have been widely used for mastitis treatment in dairy cattle. However, the use of antibiotics results in the presence of antibiotic residues in milk, which is used for human consumption. Nisin A, a bacteriocin produced by Lactococcus lactis, has been used as a broad-spectrum food preservative for over 50 years. Therefore, a combination of CEZ and nisin A might provide an extended activity spectrum against mastitis pathogens and reduce the antibiotic dose for mastitis treatment. This study aimed to evaluate the combined effect of CEZ and nisin A against mastitis pathogens using the checkerboard and time-kill assays. In the checkerboard assay, the CEZ-nisin A combination exhibited a synergistic effect against Staphylococcus aureus (n=20/20) and Enterococcus faecalis (n=13/18), and meanwhile exhibited a mostly additive effect against Staphylococcus intermedius (n=12/20), Streptococcus agalactiae (n=10/10), Streptococcus dysgalactiae (n=18/18), and Escherichia coli (n=14/18). There were no indifferent or antagonistic effects between CEZ and nisin A. In the time-kill assay, the CEZ-nisin A combination at 0.5 × or 1 × minimum inhibitory concentration exhibited synergistic reduction of bacterial growth by over 3 log10 colony forming units per ml relative to that observed with either antimicrobial substance alone. These results suggest that the CEZ-nisin A combination can be used for developing an intramammary infusion for mastitis treatment, with lower antibiotic concentrations than normal. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | citation: Kitazaki K, Koga S, Nagatoshi K, Kuwano K, Zendo T, Nakayama J, Sonomoto K, Ano H, Katamoto H. In vitro synergistic activities of cefazolin and nisin A against mastitis pathogens. J Vet Med Sci. 2017 Sep 12;79(9):1472-1479. doi: 10.1292/jvms.17-0180. Epub 2017 Jul 29. PMID: 28757508; PMCID: PMC5627315. |
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| 言語 | en | |||||
| 書誌情報 |
en : Journal of Veterinary Medical Science 巻 79, 号 9, p. 1472-1479, 発行日 2017 |
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| 出版者 | ||||||
| 出版者 | Japanese Society of Veterinary Science | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0916-7250 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1292/jvms.17-0180 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | © 2017 by the Japanese Society of Veterinary Science | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
