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Estrogen Regulates Mitochondrial Morphology through Phosphorylation of Dynamin-related Protein 1 in MCF7 Human Breast Cancer Cells
http://hdl.handle.net/10458/0002000373
http://hdl.handle.net/10458/000200037362dcb632-12e1-4149-9b7a-edc9e037bfd1
| 名前 / ファイル | ライセンス | アクション |
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article (2 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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| 公開日 | 2023-12-14 | |||||||||||||
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| タイトル | Estrogen Regulates Mitochondrial Morphology through Phosphorylation of Dynamin-related Protein 1 in MCF7 Human Breast Cancer Cells | |||||||||||||
| 言語 | en | |||||||||||||
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| 言語 | eng | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| キーワード | estrogen | |||||||||||||
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| 言語 | it | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| キーワード | cell proliferation | |||||||||||||
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| 資源タイプ | journal article | |||||||||||||
| 著者 |
ピュー, シン ウー
× ピュー, シン ウー× 山口, 優也× 澤口, 朗× ミャ, ティン チュ チョウ× チヨウジヨウフ, ナランツオツク
WEKO
26967
× Mohmand, Noor Ali× スリソワナ, ナパリ× Hino, Shin-ichiro× 菱川, 善隆 |
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Estrogen affects mitochondrial function in various tissues, but the precise mechanism remains unclear. We, therefore investigated the effect on estrogen-regulated mitochondrial morphology by dynamin-related protein 1 (Drp1) and its Ser616-phosphorylated derivative (pDrp1Ser616) are involved in mitochondrial fission. MCF7 human breast cancer cells were treated with 17β-estradiol (E2), an estrogen receptor (ER) α and β antagonist (ICI 182, 780), an ERα antagonist (MPP), and an ERβ antagonist (PHTPP) for 24 hr. The expression of Drp1 and pDrp1Ser616 was analyzed by western blotting and immunohistochemistry. Mitochondrial morphology was analyzed by transmission electron microscopy (TEM). In control cells, Drp1 was detected in the cytoplasm of all cells while pDrp1 was observed in the cytoplasm of 3.4 ± 1.0% of the total population. After E2 treatment, pDrp1Ser616-positive cells comprised 30.6 ± 5.6% of the total population, 10.5 ± 1.7% after E2 + ICI treatment, 12.4 ± 4.2% after E2 + MPP treatment, and 24.0 ± 2.2% after E2 + PHTPP treatment. In ERα knockdown MCF7 cells, pDrp1 expression was decreased after E2 treatment compared to E2-treated wild type cells. Tubular pattern mitochondria were found in the control cells but the number of short and small pattern mitochondria (< 0.5 μm2) was significantly increased after E2 treatment (as observed by TEM). We, therefore concluded that the phosphorylation of Drp1 is important for E2-dependent mitochondrial morphological changes through ERα. | |||||||||||||
| 言語 | en | |||||||||||||
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| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | Citation:Oo PS, Yamaguchi Y, Sawaguchi A, Tin Htwe Kyaw M, Choijookhuu N, Noor Ali M, Srisowanna N, Hino SI, Hishikawa Y. Estrogen Regulates Mitochondrial Morphology through Phosphorylation of Dynamin-related Protein 1 in MCF7 Human Breast Cancer Cells. Acta Histochem Cytochem. 2018 Feb 27;51(1):21-31. doi: 10.1267/ahc.17034. Epub 2018 Feb 21. PMID: 29622847; PMCID: PMC5880800. | |||||||||||||
| 言語 | en | |||||||||||||
| 書誌情報 |
en : ACTA HISTOCHEMICA ET CYTOCHEMICA 巻 51, 号 1, p. 21-31, 発行日 2018-02-27 |
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| 出版者 | ||||||||||||||
| 出版者 | The Japan Society of Histochemistry and Cytochemistry | |||||||||||||
| 言語 | en | |||||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 0044-5991 | |||||||||||||
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| 関連タイプ | isVersionOf | |||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | https://doi.org/10.1267/ahc.17034 | |||||||||||||
| 権利 | ||||||||||||||
| 言語 | en | |||||||||||||
| 権利情報 | © 2018 The Japan Society of Histochemistry and Cytochemistry | |||||||||||||
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| 出版タイプ | VoR | |||||||||||||
