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A pooled testing system to rapidly identify cattle carrying the elite controller BoLA‐DRB3*009:02 haplotype against bovine leukemia virus infection
http://hdl.handle.net/10458/0002000320
http://hdl.handle.net/10458/00020003202dba6a37-b4b8-4b81-a135-8d138e46ffb0
| 名前 / ファイル | ライセンス | アクション |
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HLA - 2021 - Notsu - A pooled testing system to rapidly identify cattle carrying the elite controller BoLA‐DRB3 009 02.pdf (4.6 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2023-11-09 | |||||
| タイトル | ||||||
| タイトル | A pooled testing system to rapidly identify cattle carrying the elite controller BoLA‐DRB3*009:02 haplotype against bovine leukemia virus infection | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | allele-specific PCR | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | BoLA-DRB3*009:02 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | bovine leukemia virus | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | elite controllers | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | pooledsamples | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | TaqMan assay | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
野津, 昂亮
× 野津, 昂亮× Hala, Gamal Ali Ali El Daous× 三苫, 修也× 乗峰, 潤三× 関口, 敏 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | As genetically resistant individuals, the “elite controllers” (ECs) of human immunodeficiency virus infection have been focused on as the keys to developing further functional treatments in medicine. In the livestock production field, identifying the ECs of bovine leukemia virus (BLV) infection in cattle is desired to stop BLV transmission chains on farms. Cattle carrying the bovine leukocyte antigen (BoLA)-DRB3*009:02 allele (DRB3*009:02) have a strong possibility of being BLV ECs. Most of cattle carrying this allele maintain undetectable BLV proviral loads and do not shed virus even when infected. BLV ECs can act as transmission barriers when placed between uninfected and infected cattle in a barn. To identify cattle carrying DRB3*009:02 in large populations more easily, we developed a pooled testing system. It employs a highly sensitive, specific real-time PCR assay and TaqMan MGB probes (DRB3*009:02-TaqMan assay). Using this system, we determined the percentage of DRB3*009:02-carrying cattle on Kyushu Island, Japan. Our pooled testing system detected cattle carrying the DRB3*009:02 allele from a DNA pool containing one DRB3*009:02-positive animal and 29 cattle with other alleles. Its capacity is sufficient for herd-level screening for DRB3*009:02-carrying cattle. The DRB3*009:02-TaqMan assay showed high-discriminative sensitivity and specificity toward DRB3*009:02, making it suitable for identifying DRB3*009:02-carrying cattle in post-screening tests on individuals. We determined that the percentage of DRB3*009:02-carrying cattle in Kyushu Island was 10.56%. With its ease of use and reliable detection, this new method strengthens the laboratory typing for DRB3*009:02-carrying cattle. Thus, our findings support the use of BLV ECs in the field. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Citation: Notsu K, El Daous H, Mitoma S, Norimine J, Sekiguchi S. A pooled testing system to rapidly identify cattle carrying the elite controller BoLA-DRB3*009:02 haplotype against bovine leukemia virus infection. HLA. 2022 Jan;99(1):12-24. doi: 10.1111/tan.14502. Epub 2021 Dec 19. PMID: 34837483; PMCID: PMC9543338. |
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| 言語 | en | |||||
| 書誌情報 |
en : HLA 巻 99, 号 1, p. 12-24, 発行日 2021-11-27 |
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| 出版者 | ||||||
| 出版者 | Wiley | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2059-2302 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1111/tan.14502 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | © 2021 The Authors. HLA: Immune Response Genetics published by John Wiley & Sons Ltd. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
