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Identifying Pathogen and Allele Type Simultaneously in a Single Well Using Droplet Digital PCR
http://hdl.handle.net/10458/0002000319
http://hdl.handle.net/10458/00020003199db2335e-5ee5-49be-9003-e1fb6ca052da
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
本文 (1.6 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2023-11-09 | |||||
| タイトル | ||||||
| タイトル | Identifying Pathogen and Allele Type Simultaneously in a Single Well Using Droplet Digital PCR | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | diagnostic | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | digital PCR | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | bovine leukemia virus | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | enzootic bovine leukosis | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | viral load quantification | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | major histocompatibility complex | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | allele-specific PCR | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | elite controller | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | superspreader | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | BoLA-DRB3*009:02, | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | BoLA-DRB3*016:01 | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
野津, 昂亮
× 野津, 昂亮× Hala, Gamal Ali Ali El Daous× 三苫, 修也× Xinyue, Wu× 乗峰, 潤三× 関口, 敏 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In the transmission control of chronic and untreatable livestock diseases such as bovine leukemia virus (BLV) infection, the removal of viral superspreaders is a fundamental approach. On the other hand, selective breeding of cattle with BLV-resistant capacity is also critical for reducing the viral damage to productivity by keeping infected cattle. To provide a way of measuring BLV proviral load (PVL) and identifying susceptible/resistant cattle simply and rapidly, we developed a fourplex droplet digital PCR method targeting the BLV pol gene, BLV-susceptible bovine major histocompatibility complex (BoLA)-DRB3*016:01 allele, resistant DRB3*009:02 allele, and housekeeping RPP30 gene (IPATS-BLV). IPATS-BLV successfully measured the percentage of BLV-infected cells and determined allele types precisely. Furthermore, it discriminated homozygous from heterozygous carriers. Using this method to determine the impact of carrying these alleles on the BLV PVL, we found DRB3*009:02-carrying cattle could suppress the PVL to a low or undetectable level, even with the presence of a susceptible heterozygous allele. Although the population of DRB3*016:01-carrying cattle showed significantly higher PVLs compared with cattle carrying other alleles, their individual PVLs were highly variable. Because of the simplicity and speed of this single-well assay, our method has the potential of being a suitable platform for the combined diagnosis of pathogen level and host biomarkers in other infectious diseases satisfying the two following characteristics of disease outcomes: (i) pathogen level acts as a critical maker of disease progression; and (ii) impactful disease-related host genetic biomarkers are already identified. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Citation: Notsu K, El Daous H, Mitoma S, Wu X, Norimine J, Sekiguchi S. Identifying Pathogen and Allele Type Simultaneously in a Single Well Using Droplet Digital PCR. mSphere. 2023 Feb 21;8(1):e0049322. doi: 10.1128/msphere.00493-22. Epub 2023 Jan 10. PMID: 36625728; PMCID: PMC9942588. |
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| 言語 | en | |||||
| 書誌情報 |
en : mSphere 巻 8, 号 1, p. e0049322, 発行日 2023-01-10 |
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| 出版者 | ||||||
| 出版者 | American Society for Microbiology | |||||
| 言語 | en | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1128/msphere.00493-22 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | © 2023 Notsu et al. This is an openaccess article distributed under the terms of the Creative Commons Attribution 4.0 International license. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
