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Development of pooled testing system for porcine epidemic diarrhoea using real-time fluorescent reverse-transcription loop-mediated isothermal amplification assay
http://hdl.handle.net/10458/0002000117
http://hdl.handle.net/10458/000200011770de1ad4-93a3-4355-b2c4-3738781476ba
| 名前 / ファイル | ライセンス | アクション |
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本文 (833 KB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2023-10-25 | |||||
| タイトル | ||||||
| タイトル | Development of pooled testing system for porcine epidemic diarrhoea using real-time fluorescent reverse-transcription loop-mediated isothermal amplification assay | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | PEDV | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | RtF-RT-LAMP | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | One-step RT-PCR | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | Pooled stool samples | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| 著者 |
マイ, ティ ガン
× マイ, ティ ガン× Nguyen, Van Diep× Yamazaki, Wataru× 岡林, 環樹× 三苫, 修也× 野津, 昂亮× Sakai, Yuta× 山口, 良二× 乗峰, 潤三× 関口, 敏 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background Porcine epidemic diarrhoea (PED) is an emerging disease in pigs that causes massive economic losses in the swine industry, with high mortality in suckling piglets. Early identification of PED virus (PEDV)-infected herd through surveillance or monitoring strategies is necessary for mass control of PED. However, a common working diagnosis system involves identifying PEDV-infected animals individually, which is a costly and time-consuming approach. Given the above information, the thrusts of this study were to develop a real-time fluorescent reverse transcription loop-mediated isothermal amplification (RtF-RT-LAMP) assay and establish a pooled testing system using faecal sample to identify PEDV-infected herd. Results In this study, we developed an accurate, rapid, cost-effective, and simple RtF- RT-LAMP assay for detecting the PEDV genome targeting M gene. The pooled testing system using the RtF-RT-LAMP assay was optimized such that a pool of at least 15 individual faecal samples could be analysed. Conclusions The developed RtF-RT-LAMP assay in our study could support the design and implementation of large-scaled epidemiological surveys as well as active surveillance and monitoring programs for effective control of PED. |
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| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Citation: Mai, T.N., Nguyen, V., Yamazaki, W. et al. Development of pooled testing system for porcine epidemic diarrhoea using real-time fluorescent reverse-transcription loop-mediated isothermal amplification assay. BMC Vet Res 14, 172 (2018). https://doi.org/10.1186/s12917-018-1498-9 |
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| 言語 | en | |||||
| 書誌情報 |
en : BMC Veterinary Research 巻 14, 号 1, p. 172(2018), 発行日 2018-05-29 |
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| 出版者 | ||||||
| 出版者 | BioMed Central Ltd | |||||
| 言語 | en | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1186/s12917-018-1498-9 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
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マイ, ティ ガン, Nguyen, Van Diep, Yamazaki, Wataru, 岡林, 環樹, 三苫, 修也, 野津, 昂亮, Sakai, Yuta, 山口, 良二, 乗峰, 潤三, 関口, 敏, 2018, Development of pooled testing system for porcine epidemic diarrhoea using real-time fluorescent reverse-transcription loop-mediated isothermal amplification assay: BioMed Central Ltd, 172(2018)– p.
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