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Ecotropic viral integration site 1 regulates EGFR transcription in glioblastoma cells
http://hdl.handle.net/10458/0002000104
http://hdl.handle.net/10458/0002000104912699d9-2c08-49c4-a6a7-c86e97ed9793
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Mizuguchi-Asako_11060_2019_Article_3310.pdf (4.9 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2023-10-24 | |||||
| タイトル | ||||||
| タイトル | Ecotropic viral integration site 1 regulates EGFR transcription in glioblastoma cells | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | Ecotropic viral integration site-1 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | Epidermal growth factor receptor | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | Glioblastoma | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | Transcription factor | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | Tyrosine kinase receptor | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
水口, 麻子
× 水口, 麻子× 山下, 真治× 横上, 聖貴× 森下, 和広× 竹島, 秀雄 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Purpose Ecotropic viral integration site-1 (EVI1) is a transcription factor that contributes to the unfavorable prognosis of leukemia, some epithelial cancers, and glial tumors. However, the biological function of EVI1 in glioblastoma multiforme (GBM) remains unclear. Based on microarray experiments, EVI1 has been reported to regulate epidermal growth factor receptor (EGFR) transcription. Signal transduction via EGFR plays an essential role in glioblastoma. Therefore, we performed this study to clarify the importance of EVI1 in GBM by focusing on the regulatory mechanism between EVI1 and EGFR transcription. Methods We performed immunohistochemical staining and analyzed the EVI1-expression in glioma tissue. To determine the relationship between EVI1 and EGFR, we induced siRNA-mediated knockdown of EVI1 in GBM cell lines. To investigate the region that was essential for the EVI1 regulation of EGFR expression, we conducted promoter reporter assays. We performed WST-8 assay to investigate whether EVI1 affected on the proliferation of GBM cells or not. Results It was observed that 22% of GBM tissues had over 33% of tumor cells expressing EVI1, whereas no lower-grade glioma tissue had over 33% by immunohistochemistry. In A172 and YKG1 cells, the expression levels of EGFR and EVI1 correlated. Analysis of the EGFR promoter region revealed that the EGFR promoter (from − 377 to − 266 bp) was essential for the EVI regulation of EGFR expression. We showed that EVI1 influenced the proliferation of A172 and YKG1 cells. Conclusion This is the first study reporting the regulation of EGFR transcription by EVI1 in GBM cells. |
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| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Citation: Mizuguchi, A., Yamashita, S., Yokogami, K. et al. Ecotropic viral integration site 1 regulates EGFR transcription in glioblastoma cells. J Neurooncol 145, 223–231 (2019). https://doi.org/10.1007/s11060-019-03310-z |
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| 言語 | en | |||||
| 書誌情報 |
en : Journal of Neuro-Oncology 巻 145, 号 2, p. 223-231, 発行日 2019-10-15 |
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| 出版者 | ||||||
| 出版者 | Springer Nature | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0167-594X | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1007/s11060-019-03310-z | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
