| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2023-09-04 |
| タイトル |
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タイトル |
Relationship between Allelic Heterozygosity in BoLA-DRB3 and Proviral Loads in Bovine Leukemia Virus-Infected Cattle |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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キーワード |
BoLA-DRB3 allele combinations |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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キーワード |
bovine leukemia virus |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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キーワード |
heterozygous alleles |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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キーワード |
proviral load |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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キーワード |
RFLP-PCR |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
Daous, Hala El
三苫, 修也
Elhanafy Eslam
Thi Nguyen, Huyen
Thi Mai, Ngan
野津, 昂亮
Kaneko, Chiho
乗峰, 潤三
関口, 敏
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Enzootic bovine leukosis is a lethal neoplastic disease caused by bovine leukemia virus (BLV), belongs to family Retroviridae. The BLV proviral load (PVL) represents the quantity of BLV genome that has integrated into the host’s genome in BLV-infected cells. Bovine leukocyte antigen (BoLA) class II allelic polymorphisms are associated with PVLs in BLV-infected cattle. We sought to identify relationships between BoLA-DRB3 allelic heterozygosity and BLV PVLs among different cattle breeds. Blood samples from 598 BLV-infected cattle were quantified to determine their PVLs by real-time polymerase chain reaction. The results were confirmed by a BLV-enzyme-linked immunosorbent assay. Restriction fragment length polymorphism-polymerase chain reaction identified 22 BoLA-DRB3 alleles. Multivariate negative binomial regression modeling was used to test for associations between BLV PVLs and BoLA-DRB3 alleles. BoLA-DRB3.2*3, *7, *8, *11, *22, *24, and *28 alleles were significantly associated with low PVLs. BoLA-DRB3.2*10 was significantly associated with high PVLs. Some heterozygous allele combinations were associated with low PVLs (*3/*28, *7/*8, *8/*11, *10/*11, and *11/*16); others were associated with high PVLs (*1/*41, *10/*16, *10/*41, *16/*27, and *22/*27). Interestingly, the BoLA-DRB3.2*11 heterozygous allele was always strongly and independently associated with low PVLs. This is the first reported evidence of an association between heterozygous allelic combinations and BLV PVLs. |
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言語 |
en |
| 内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
Citation: Daous, H.E.; Mitoma, S.; Elhanafy, E.; Thi Nguyen, H.; Thi Mai, N.; Notsu, K.; Kaneko, C.; Norimine, J.; Sekiguchi, S. Relationship between Allelic Heterozygosity in BoLA-DRB3 and Proviral Loads in Bovine Leukemia Virus-Infected Cattle. Animals 2021, 11, 647. https://doi.org/10.3390/ani11030647 |
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言語 |
en |
| 書誌情報 |
en : Animals
巻 11,
号 3,
p. 647
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| 出版者 |
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出版者 |
MDPI |
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言語 |
en |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.3390/ani11030647 |
| 権利 |
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言語 |
en |
|
権利情報 |
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
| 著者版フラグ |
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出版タイプ |
VoR |
Fulltext (12.3 MB)
