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Relationship between copper biosorption and microbial inhibition of hydroxyl radical formation in a copper(II)–hydrogen peroxide system
http://hdl.handle.net/10458/1011
http://hdl.handle.net/10458/1011f5464134-b600-4981-ab37-af3747ff6388
| 名前 / ファイル | ライセンス | アクション |
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WorldJMicrobiolBiotechnol-inpress.pdf (232.2 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2007-12-05 | |||||
| タイトル | ||||||
| タイトル | Relationship between copper biosorption and microbial inhibition of hydroxyl radical formation in a copper(II)–hydrogen peroxide system | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| キーワード | copper biosorption, Cu(II) ion, DMPO, DMPO-OH, hydrogen peroxide, hydroxyl radical elimination | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Nakajima, Akira
× Nakajima, Akira× Ueda, Yuto |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The microbial retardation of the spin adduct, DMPO-OH, formed in a copper（II）- hydrogen peroxide-DMPO （5,5-dimethyl-1-pyrroline N-oxide） solution was examined in relation to copper biosorption. A hydroxyl radical is formed in the solution through two steps, the reduction of Cu（II） to Cu（I） by H2O2 and the Fenton-type reaction of Cu（I） with H2O2. The resultant radical is trapped by DMPO to form DMPO-OH. Microbial cells retarded the DMPO-OH in the Cu（II）-H2O2-DMPO far more significantly than in the UV-irradiated H2O2- DMPO solution. Egg albumin showed a higher DMPO-OH retardation than microbial cells both in the Cu（II）-H2O2-DMPO and the UV-irradiated H2O2-DMPO solutions. These results indicated that the retardation effect is related to organic matter and not to microbial activity. Microorganisms having higher affinities for copper ion retarded DMPO-OH more significantly. The linear relationship between the amounts of copper biosorption and the inverse of the median inhibitory doses for DMPO-OH indicated that the microbial cells inhibited the reduction of Cu（II） to Cu（I） by H2O2, followed by the decrease of hydroxyl radical formation and the retardation of DMPO-OH. These results also suggest that the coupling between microbial cells and Cu（II） ion can be estimated from their ability to retard DMPO-OH. |
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| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The original publication is available at www.springerlink.com | |||||
| 言語 | en | |||||
| 書誌情報 |
en : World journal of microbiology & biotechnology 発行日 2007-11-15 |
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| 出版者 | ||||||
| 出版者 | Springer | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 09593993 | |||||
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| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10811107 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
