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Loss of ribosomal RNA modification causes developmental defects in zebrafish.
http://hdl.handle.net/10458/3669
http://hdl.handle.net/10458/3669c21ed5ed-0c36-4958-8236-229e0e87f764
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
kenmochi_Nucleic Acids Res .pdf (3.2 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2012-05-22 | |||||
| タイトル | ||||||
| タイトル | Loss of ribosomal RNA modification causes developmental defects in zebrafish. | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Higa-Nakamine, Sayomi
× Higa-Nakamine, Sayomi× Suzuki, Takeo× Uechi, Tamayo× Chakraborty, Anirban× Nakajima, Yukari× Nakamura, Mikako× Hirano, Naoko× Suzuki, Tsutomu× Kenmochi, Naoya |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Non-coding RNAs （ncRNAs） play key roles in diverse cellular activities, and efficient ncRNA function requires extensive posttranscriptional nucleotide modifications. Small nucleolar RNAs （snoRNAs） are a group of ncRNAs that guide the modification of specific nucleotides in ribosomal RNAs （rRNAs） and small nuclear RNAs. To investigate the physiological relevance of rRNA modification in vertebrates, we suppressed the expression of three snoRNAs （U26, U44 and U78）, either by disrupting the host gene splicing or by inhibiting the snoRNA precursor processing, and analyzed the consequences of snoRNA loss-of-function in zebrafish. Using a highly sensitive mass spectrometric analysis, we found that decreased snoRNA expression reduces the snoRNA-guided methylation of the target nucleotides. Impaired rRNA modification, even at a single site, led to severe morphological defects and embryonic lethality in zebrafish, which suggests that rRNA modifications play an essential role in vertebrate development. This study highlights the importance of posttranscriptional modifications and their role in ncRNA function in higher eukaryotes. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Nucleic acids research 巻 40, 号 1, p. 391-398, 発行日 2012-01 |
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| 出版者 | ||||||
| 出版者 | Oxford University Press | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 13624962 | |||||
| PubMed番号 | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 21908402 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
