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βアミロイドタンパクによる培養細胞系でのラジカル発生
http://hdl.handle.net/10458/2137
http://hdl.handle.net/10458/21379dd894d3-6647-4a71-a5c3-8197c4d7579e
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
4-2b_ueda-jiki-vol.13-2002.pdf (357.1 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2009-08-04 | |||||
| タイトル | ||||||
| タイトル | βアミロイドタンパクによる培養細胞系でのラジカル発生 | |||||
| 言語 | ja | |||||
| タイトル | ||||||
| タイトル | Free radical generation from PC12 cells by beta amyloid peptide stimulation | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 言語 | en | |||||
| キーワード | Beta amyloid peptide, PC12 cell, lipid peroxidation, hydroxyl radical, lipid radical | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
林, 要人
× 林, 要人× 植田, 勇人× 中島, 暉× 横山, 秀克× 大矢, 博明× 鎌田, 仁× 三山, 吉夫× 林, 要人 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | There is few direct electron paramagnetic resonance （EPR） evidence of supporting the hypothesis of lipid peroxidation induced by beta amyloid. Hence in this study EPR measurement was performed in the cultured PC12 cells using two kinds of spin trapping agents （DMPO, POBN） to test the hypothesis. Spin adduct of DMPO in the cells stimulated by beta amyloid （25-35 400uM） revealed characteristically four line spectra, for which the hfc were aN=14.9G and aH=14.9G; its hfc were corresponded to DMPO-OH. The EPR signal from the PC12 cells cultured with beta amyloid were characteristically six-line spectra, for which the hfc were aN=15. 7G and aH=2.5G; its hfc were corresponded to that of lipid radical. This study presents the direct EPR evidence of hydroxyl radical formation in PC12 cell cultured with beta amyloid 25-35 peptide. Generation of hydroxyl radical results in abstraction hydrogen from polyunsaturated lipid acid, and plays a causative role in lipid peroxidation. This result might be important evidence of initiation of lipid peroxidation and the growing lipid peroxidation in the cascade of neurotoxicity induced by beta amyloid 25-35 peptide. | |||||
| 言語 | en | |||||
| 書誌情報 |
ja : 磁気共鳴と医学 巻 13, p. 55-58, 発行日 2002 |
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| 出版者 | ||||||
| 出版者 | 日本医学館 | |||||
| 言語 | ja | |||||
| 出版者 | ||||||
| 出版者 | Magnetic resonance in medicine | |||||
| 言語 | en | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN10217072 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
