| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2008-07-14 |
| タイトル |
|
|
タイトル |
Development of ET, primary myelofibrosis, and PV in mice expressing JAK2 V617F |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
myeloproliferative diseases, JAK2 V617F, transgenic mice |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| その他（別言語等）のタイトル |
|
|
その他のタイトル |
Running title: JAK2 V617F causes classical MPDs |
|
言語 |
en |
| 著者 |
幣, 光太郎
下田, 晴子
Kumano, Takashi
Karube, Kennosuke
亀田, 拓郎
Takenaka, Katsuto
Oku, Seido
安倍, 弘生
Katayose, Keiko S
久冨木, 庸子
楠元, 寿典
蓮池, 悟
田原, 義弘
永田, 賢治
Matsuda, Tadashi
Ohshima, Koichi
Harada, Mine
下田, 和哉
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
An acquired JAK2 V617F mutation is found in most patients with polycythemia vera （PV）, and about half of patients with essential thrombocythemia （ET） or primary myelofibrosis （PMF）. Mice transplanted with bone marrow cells in which JAK2 V617F was retrovirally expressed developed PV-like features, but not ET or PMF. To address the contribution of this mutation to the pathogenesis of these three MPDs, we generated two lines of JAK2 V617F transgenic mice. One line showed granulocytosis after 4 months of age. Among 43 mice, 8 （19%） showed polycythemia and 15 （35%） showed thrombocythemia. The second line showed extreme leukocytosis and thromobocytosis. They showed anemia that means Hb value from 9 to 10 g/dL at one month old. Myeloid cells and megakaryocytes were predominant in the bone marrow of these animals, and splenomegaly was observed. The expression of JAK2 V617F mRNA in bone marrow cells was 0.45 and 1.35 that of endogenous wild-type JAK2 in the two lines. In vitro analysis of bone marrow cells from both lines showed constitutive activation of ERK1/2, STAT5, and AKT, and augmentation of their phosphorylations by cytokine stimulation. We conclude that in vivo expression of JAK2 V617F results in ET-like, PMF-like, and PV-like disease. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
http://www.nature.com/leu/journal |
|
言語 |
en |
| 書誌情報 |
en : Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K.
巻 22,
号 1,
p. 87-95,
発行日 2008
|
| 出版者 |
|
|
出版者 |
Nature Publishing Group |
|
言語 |
en |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
08876924 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10668706 |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
shimoda leukemia 22(1)2008.pdf (1.5 MB)
