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Presence of protein phosphatase type 1 and its involvement in temperature-dependent flagellar movement of fowl spermatozoa
http://hdl.handle.net/10458/709
http://hdl.handle.net/10458/70913b49fd2-4427-40dc-a642-cd9e8b39506d
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ashizawa47.pdf (892.2 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2007-10-10 | |||||
| タイトル | ||||||
| タイトル | Presence of protein phosphatase type 1 and its involvement in temperature-dependent flagellar movement of fowl spermatozoa | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | ja | |||||
| 主題Scheme | Other | |||||
| キーワード | 精子 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | sperm, phosphorylation, dephosphorylation, protein phosphatase, Okadaic acid, Calyculin A, motility, spermatozoa | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
芦沢, 幸二
× 芦沢, 幸二× Wishart, Graham J.× Tomonaga, Hiroshi× Nishinakama, Kohfumi× 續木, 靖浩 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Even in the presence of ATP, the motility of demembranated fowl spermatozoa was negligible at the avian body temperature of 40°C. Motility could be restored by the addition of calyculin A, okadaic acid, inhibitor 1 or inhibitor 2, which are specific inhibitors of phosphatase type 1 （PP1）. Demembranated spermatozoa, stimulated by calyculin A or okadaic acid, lost their motility following the addition of 1 mM CaCl2, but this was restored gradually by the stepwise addition of EGTA. Immunoblotting of sperm extract using an antibody to PP1 revealed a major cross-reacting protein of 36-37 kDa, which corresponded to the molecular weight of the known catalytic subunit of PP1. These results suggest that PP1 present in the fowl sperm axoneme may be involved in the inhibition of fowl sperm motility at 40°C via Ca2+-dependent regulatory systems. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Journal HP: http://www.febsletters.com/ | |||||
| 言語 | en | |||||
| 書誌情報 |
en : FEBS letters : for the rapid publication of short reports in biochemistry, biophysics and molecular biology 巻 350, 号 1, p. 130-134, 発行日 1994 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00145793 | |||||
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| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00642943 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
