{"created":"2023-07-29T13:00:13.128365+00:00","links":{},"metadata":{"_buckets":{"deposit":"4cb66dd3-7331-418e-b7e9-3f971f7b5862"},"_deposit":{"created_by":9,"id":"849.3","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"849.3"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00000849.3","sets":["74","74:26"]},"author_link":["932","5389","5390","2104"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2000","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"104","bibliographicPageStart":"99","bibliographicVolumeNumber":"56","bibliographic_titles":[{"bibliographic_title":"Molecular reproduction and development","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The motility of intact fowl spermatozoa was vigorous at 25°C, but decreased gradually following the addition of 0-100 μM β-carotene in a dose-dependent manner. Even in the presence of stimulators of fowl sperm motility, such as Ca2+ or calyculin A, the motility of intact spermatozoa at both 25°C and 40°C remained inhibited following the addition of β- carotene. Under all of these circumstances, sperm ATP concentrations were not reduced by the addition of β-carotene. Moreover, the motility of demembranated spermatozoa was not inhibited by the addition of the same concentrations of β-carotene. No changes in intracellular free Ca2+ concentrations, measured by means of a fluorescent Ca2+ indicator, fura-2, were observed in intact β-carotene -treated spermatozoa. These results suggest that β-carotene is involved in the inhibition of the flagellar movement of fowl spermatozoa without change in energy production, and that the target of β-carotene might be present in the cytoplasmic matrix and/or the plasma membrane, but not retained in the axoneme and/or accessory cytoskeletal components.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10735246","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1040452X","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"芦沢, 幸二","creatorNameLang":"ja"},{"creatorName":"Ashizawa, Koji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"932","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tomita, Yoshifumi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"5389","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kanemaru, Chikako","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"5390","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsuzuki, Yasuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2104","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00236928","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00236928"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"ashizawa73.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ashizawa73.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/849/files/ashizawa73.pdf"},"version_id":"053ae1bc-f1b7-4199-ab7f-40a9e1c46fd1"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"精子","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"sperm, Spermatozoa, β-carotene, Motility, ATP concentrations, Intracellular free calcium concentrations","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"In vitro Effects of β-Carotene for the Motility, ATP and Intracellular Free Ca2+ Concentrations of Fowl Spermatozoa","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"In vitro Effects of β-Carotene for the Motility, ATP and Intracellular Free Ca2+ Concentrations of Fowl Spermatozoa","subitem_title_language":"en"},{"subitem_title":"β-carotene and fowl sperm function","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"5","path":["74","26"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-10-11"},"publish_date":"2007-10-11","publish_status":"0","recid":"849.3","relation_version_is_last":true,"title":["In vitro Effects of β-Carotene for the Motility, ATP and Intracellular Free Ca2+ Concentrations of Fowl Spermatozoa"],"weko_creator_id":"5","weko_shared_id":-1},"updated":"2023-07-29T13:00:15.632301+00:00"}