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Inhibition of Flagellar Motility of Fowl Spermatozoa by L-Carnitine: Its Relationship with Respiration and Phosphorylation of Axonemal Proteins
http://hdl.handle.net/10458/733
http://hdl.handle.net/10458/73311ff8060-239f-4bdf-a745-8bf4cc8f28d9
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ashizawa50.pdf (1.6 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2007-10-11 | |||||
| タイトル | ||||||
| タイトル | Inhibition of Flagellar Motility of Fowl Spermatozoa by L-Carnitine: Its Relationship with Respiration and Phosphorylation of Axonemal Proteins | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | ja | |||||
| 主題Scheme | Other | |||||
| キーワード | 精子 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | sperm, Demembranated sperm, Dynein, Calcium, Oxygen consumption | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| その他（別言語等）のタイトル | ||||||
| その他のタイトル | Carnitine and sperm motility | |||||
| 言語 | en | |||||
| 著者 |
芦沢, 幸二
× 芦沢, 幸二× Kamiya, Tetsushi× Tamura, Ikue× 續木, 靖浩 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The action of carnitine in regulating fowl sperm motility was investigated. As the concentration of L-carnitine was increased (0-20 mM), the motility of intact and demembranated fowl spermatozoa was reduced at 30°C. Even the presence of 1 mM CaCl2 before the addition of 10 mM carnitine could not prevent the inhibition of motility at 30°C and 40°C. However, motility was restored by reducing the concentrations of carnitine. Carnitine also inhibited the oxygen consumption and ATP concentrations of intact spermatozoa, and caused a reduction in intracellular free Ca2+ concentrations. Phosphorylation of a 50 kDa protein and dephosphorylation of 24 kDa and 30 kDa proteins of demembranated spermatozoa were observed after the addition of carnitine. In contrast, the flagellar ATPase activity of crude dynein extract was not affected by the addition of carnitine. These results suggest that inhibitory effect of carnitine for motility may be directly on the axonemal phosphoproteins, but not directly on the dynein ATPase activity. The physiological role of carnitine for fowl spermatozoa in the ductus deferens is discussed. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Molecular reproduction and development 巻 38, 号 3, p. 318-325, 発行日 1994 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1040452X | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10735246 | |||||
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| 出版タイプ | AM | |||||
