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MAINTENANCE OF MOTILITY OF BULL SPERMATOZOA BY A LOW MOLECULAR WEIGHT FACTOR(S) RELEASED FROM CULTURED EMBRYONIC CELLS
http://hdl.handle.net/10458/737
http://hdl.handle.net/10458/73704c8b2bb-42a0-4b4a-928a-3d3b71680f90
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ashizawa19.pdf (662.2 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2007-10-11 | |||||
| タイトル | ||||||
| タイトル | MAINTENANCE OF MOTILITY OF BULL SPERMATOZOA BY A LOW MOLECULAR WEIGHT FACTOR(S) RELEASED FROM CULTURED EMBRYONIC CELLS | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | ja | |||||
| 主題Scheme | Other | |||||
| キーワード | 精子 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | sperm | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
芦沢, 幸二
× 芦沢, 幸二× Ichinari, Yuko× Yoshidome, Shinya× Okauchi, Keizo |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | This study was undertaken to investigate the factor（s） released from cultured embryonic cells that is responsible for the prolonged motility of bull spermatozoa and to determine some of the properties of the factor（s）. Spermatozoa were incubated at 37°C in a supernatant removed from a 4-day culture medium of embryonic chick skeletal muscle cells. Their motility was maintained for 25.2 h. In contrast, spermatozoa incubated in the fresh medium rapidly lost their motility. A filtrate of the 4-day culture medium, obtained by passing the fluid through an Amicon YC-05 ultrafiltration membrane, retained a favorable effect on prolonging motility of spermatozoa. Heating or freeze-thawing of the filtrate did not interfere with the ability of the spermatozoa to maintain their motility. Oxygen consumption of spermatozoa in the filtrate of the 4-day culture medium was similar to that of spermatozoa in the fresh medium. These results suggest that a low molecular weight factor（s） （Mol. Wt. < 500） supplied by the cultured cells effectively prolonged the survival of the spermatozoa. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Animal reproduction science 巻 10, 号 1, p. 185-192, 発行日 1986 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 03784320 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00525633 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
