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Inhibition of temperature-dependent immobilization of fowl spermatozoa at body temperature by an increased intracellular pH
http://hdl.handle.net/10458/708
http://hdl.handle.net/10458/70863bbaedc-31d8-4371-b775-6fa4026792ed
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ashizawa46.pdf (1.5 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2007-10-10 | |||||
| タイトル | ||||||
| タイトル | Inhibition of temperature-dependent immobilization of fowl spermatozoa at body temperature by an increased intracellular pH | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | ja | |||||
| 主題Scheme | Other | |||||
| キーワード | 精子 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | sperm, spermatozoa, motility, intracellular pH, temperature, fowl | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| その他（別言語等）のタイトル | ||||||
| その他のタイトル | Intracellular pH and fowl sperm motility | |||||
| 言語 | en | |||||
| 著者 |
芦沢, 幸二
× 芦沢, 幸二× Wishart, G.J.× Nakao, H.× Okino, Y.× 續木, 靖浩 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Fowl sperm motility was measured by altering the extracellular pH (pH(e)) at 30°C and 40°C. At 30°C, the motility of intact spermatozoa was vigorous in a medium in the wide pH(e) range of 7.3-10.1. In contrast, intact spermatozoa were almost immotile at 40°C in a medium below pH(e) 8.1. However, the motility could be restored by increasing the pH(e). Maximum motility (67%) was obtained in medium at pH(e) 9.4. Stimulation of the motility of demembranated spermatozoa was also observed with an increased pH(e) at 40°C. However, demembranated spermatozoa, stimulated by increasing the pH(e) at 40°C , lost their motility by the addition of 1 mmol CaCl2 l-1. Motility was restored by the subsequent addition of 2 mmol EGTA l-1. Under these conditions, the flagellar ATPase activity of crude dynein extract was not affected regardless of the addition of CaCl2 or EGTA. The intracellular pH (pH(i)) of intact spermatozoa, estimated by measuring the accumulation of 9-aminoacridine fluorescence, elevated with an increased pH (e) at both 30°C and 40°C, although at the same pH(e) value, acidification of pH(i) by 0.25-0.30 pH units was observed at 40°C compared with at 30°C. These results demonstrate that the reversible temperature-dependent immobilization of fowl spermatozoa at 40°C is inhibited by an increased pH(i). Furthermore, it is possible that the action of the increased pH(i) may not be directly on dynein ATPase activity, but rather mediated by a Ca2+-related substance(s) on the axoneme. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Disclaimer. This is not the definitive version of record of this article. This manuscript has been accepted for publication in Reproduction, but the version presented here has not yet been copy edited, formatted or proofed. Consequently, neither Reproduction, the Society for Reproduction and Fertility nor its publisher, BioScientifica Ltd, accepts responsibility for any errors or omissions it may contain. 1994 Society for Reproduction and Fertility. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Journal of reproduction and fertility 巻 101, 号 3, p. 593-598, 発行日 1994 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00224251 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00705952 | |||||
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| 出版タイプ | AM | |||||
