{"created":"2023-05-15T09:57:00.802204+00:00","id":770,"links":{},"metadata":{"_buckets":{"deposit":"b5541816-9fcc-4414-8284-68071b72fa83"},"_deposit":{"created_by":5,"id":"770","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"770"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00000770","sets":["74","74:26"]},"author_link":["4704","4712","4708","4717","16713","4707","2692","4711","4709","4706","4614","4702","2693","4716","4703","4713","4701"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-05-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"636","bibliographicPageStart":"633","bibliographicVolumeNumber":"65","bibliographic_titles":[{"bibliographic_title":"The journal of veterinary medical science","bibliographic_titleLang":"en"},{"bibliographic_title":"Journal of veterinary medical science","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Chimeric simian and human immunodeficiency viruses （SHIVs） are useful tool for investigating AIDS pathogenesis and for development of vaccine. We constructed a SHIV-vpr vector （designated as SHIV-3sj） by replacing vpr region with restriction enzyme sites. SHIV-3sj was designed to express inserted gene along with its viral replication. Five cytokine genes were inserted into SHIV-3sj, and ability of viral replication and expression of the inserted genes were examined. The short insert including RANTES and IL-5 resulted in the successful expression from SHIV-3sj, while the construct having longer genes including IL-2, IL-6 and IL-12p35 failed to become replication competent. These results suggest that the length of the insert is an important factor for the replication ability of SHIV-3sj vector.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"SIV/HIVキメラウイルス（SHIV）のvpr領域を制限酵素サイトに置換し,SHIV-3sjベクターを作成した.5種のサイトカイン遺伝子をSHIV3sjに挿入した結果,RANTESやIL-5といった短い遺伝子は発現が確認されたが,IL-2,IL-6,IL-12p35といった長い遺伝子を挿入したものは複製能を欠いた.挿入遺伝子の長さがSHIV-3sjベクターの複製を規定している可能性が示唆された.","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japanese Society of Veterinary Science","subitem_publisher_language":"en"},{"subitem_publisher":"日本獣医学会","subitem_publisher_language":"ja"}]},"item_10001_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"12808218","subitem_relation_type_select":"PMID"}}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10796138","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09167250","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"13477439","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"芳賀, 猛","creatorNameLang":"ja"},{"creatorName":"ハガ, タケシ","creatorNameLang":"ja-Kana"},{"creatorName":"Haga, Takeshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"大古場, 正史","creatorNameLang":"ja"},{"creatorName":"オオコバ, マサシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山崎, 奈々瀬","creatorNameLang":"ja"},{"creatorName":"ヤマザキ, ナナセ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"隈部, 志野","creatorNameLang":"ja"},{"creatorName":"クマベ, シノ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"清水, 佑也","creatorNameLang":"ja"},{"creatorName":"シミズ, ユウヤ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"後藤, 義孝","creatorNameLang":"ja"},{"creatorName":"ゴトウ, ヨシタカ","creatorNameLang":"ja-Kana"},{"creatorName":"Goto, Yoshitaka","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"桑田, 岳夫","creatorNameLang":"ja"},{"creatorName":"クワタ, タケオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"コズレフ, ユーリ","creatorNameLang":"ja"},{"creatorName":"コズレフ, ユーリ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"速水, 正憲","creatorNameLang":"ja"},{"creatorName":"ヤハミ, マサノリ","creatorNameLang":"ja-Kana"},{"creatorName":"Hayami, Masanori","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"三浦, 智行","creatorNameLang":"ja"},{"creatorName":"ミウラ, トモユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okoba, Masashi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamazaki, Nanase","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kumabe, Shino","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimizu, Yuya","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kuwata, Takeo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kozyrev, Iouri L.","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"速水, 正憲","creatorNameLang":"ja"},{"creatorName":"ヤハミ, マサノリ","creatorNameLang":"ja-Kana"},{"creatorName":"Hayami, Masanori","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miura, Tomoyuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"2881haga.pdf","filesize":[{"value":"62.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"2881haga.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/770/files/2881haga.pdf"},"version_id":"1f170419-7f6a-4143-9f8d-a7ecd2ab13e1"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"SHIV, vector, vpr","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characterization of vpr Vector Constructed from Chimeric Simian and Human Immunodeficiency Virus","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of vpr Vector Constructed from Chimeric Simian and Human Immunodeficiency Virus","subitem_title_language":"en"},{"subitem_title":"SIV/HIVキメラウイルスより構築されたvprベクターの性状","subitem_title_language":"ja"}]},"item_type_id":"10001","owner":"5","path":["74","26"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2010-05-26"},"publish_date":"2010-05-26","publish_status":"0","recid":"770","relation_version_is_last":true,"title":["Characterization of vpr Vector Constructed from Chimeric Simian and Human Immunodeficiency Virus"],"weko_creator_id":"5","weko_shared_id":2},"updated":"2023-10-24T01:47:43.160138+00:00"}