@article{oai:miyazaki-u.repo.nii.ac.jp:00000770,
 author = {芳賀, 猛 and Haga, Takeshi and 大古場, 正史 and 山崎, 奈々瀬 and 隈部, 志野 and 清水, 佑也 and 後藤, 義孝 and Goto, Yoshitaka and 桑田, 岳夫 and コズレフ, ユーリ and 速水, 正憲 and Hayami, Masanori and 三浦, 智行 and Okoba, Masashi and Yamazaki, Nanase and Kumabe, Shino and Shimizu, Yuya and Kuwata, Takeo and Kozyrev, Iouri L. and 速水, 正憲 and Hayami, Masanori and Miura, Tomoyuki},
 issue = {5},
 journal = {The journal of veterinary medical science, Journal of veterinary medical science},
 month = {May},
 note = {Chimeric simian and human immunodeficiency viruses （SHIVs） are useful tool for investigating AIDS pathogenesis and for development of vaccine. We constructed a SHIV-vpr vector （designated as SHIV-3sj） by replacing vpr region with restriction enzyme sites. SHIV-3sj was designed to express inserted gene along with its viral replication. Five cytokine genes were inserted into SHIV-3sj, and ability of viral replication and expression of the inserted genes were examined. The short insert including RANTES and IL-5 resulted in the successful expression from SHIV-3sj, while the construct having longer genes including IL-2, IL-6 and IL-12p35 failed to become replication competent. These results suggest that the length of the insert is an important factor for the replication ability of SHIV-3sj vector., SIV/HIVキメラウイルス（SHIV）のvpr領域を制限酵素サイトに置換し,SHIV-3sjベクターを作成した.5種のサイトカイン遺伝子をSHIV3sjに挿入した結果,RANTESやIL-5といった短い遺伝子は発現が確認されたが,IL-2,IL-6,IL-12p35といった長い遺伝子を挿入したものは複製能を欠いた.挿入遺伝子の長さがSHIV-3sjベクターの複製を規定している可能性が示唆された.},
 pages = {633--636},
 title = {Characterization of vpr Vector Constructed from Chimeric Simian and Human Immunodeficiency Virus},
 volume = {65},
 year = {2003},
 yomi = {ハガ, タケシ and オオコバ, マサシ and ヤマザキ, ナナセ and クマベ, シノ and シミズ, ユウヤ and ゴトウ, ヨシタカ and クワタ, タケオ and コズレフ, ユーリ and ヤハミ, マサノリ and ミウラ, トモユキ and ヤハミ, マサノリ}
}