{"created":"2023-05-15T09:57:00.736794+00:00","id":769,"links":{},"metadata":{"_buckets":{"deposit":"dc9a61b7-8a1f-42d0-816a-94ad7b02b99b"},"_deposit":{"created_by":5,"id":"769","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"769"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00000769","sets":["74","74:26"]},"author_link":["2692","4692","4693","4614","4695","2693","4697","4698","16713"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-07-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"409","bibliographicPageStart":"405","bibliographicVolumeNumber":"55","bibliographic_titles":[{"bibliographic_title":"Experimental Animals","bibliographic_titleLang":"en"},{"bibliographic_title":"実験動物ニュース","bibliographic_titleLang":"ja"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The ability of live attenuated Salmonella enterica serovar typhimurium （S. typhimurium） as a carrier of DNA vaccine was evaluated using model plasmid encoding beta-galactosidase （β-Gal） and BALB/c mice. We constructed pBRCMVβ, β-Gal expression apparatus having a replication origin from low copy pBR322. Comparison of the plasmid stability showed that pBRCMVβ remained stable in Salmonella even after oral administration, while pUC-based pCMVβ tended to be lost quickly. However, titers for β-Gal specific IgG in sera did not significantly increase in mice orally administered S. typhimurium harboring pBRCMVβ. These data suggest that the stability of plasmid in S. typhimurium is associated with its replication origin. Further studies are required to scientifically establish this methodology.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japanese Association for Laboratory Animal Science","subitem_publisher_language":"en"},{"subitem_publisher":"社団法人 日本実験動物学会","subitem_publisher_language":"ja"}]},"item_10001_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"16880689","subitem_relation_type_select":"PMID"}}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11032321","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13411357","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Haga, Takeshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2692","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kumabe, Shino","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4692","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ikejiri, Ai","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4693","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimizu, Yuya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4614","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Li, Huang","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4695","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Goto, Yoshitaka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2693","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsui, Hidenori","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4697","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miyata, Hironori","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4698","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miura, Tomoyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"16713","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"451-haga-Exp.Anim-vol.55-4-2006.pdf","filesize":[{"value":"709.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"451-haga-Exp.Anim-vol.55-4-2006.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/769/files/451-haga-Exp.Anim-vol.55-4-2006.pdf"},"version_id":"6c860560-67d2-4639-bbca-066e8ea9357e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"DNA vaccine, plasmid stability, Salmonella typhimurium","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"In vitro and in vivo stability of plasmids in attenuated Salmonella enterica serovar typhimurium used as a carrier of DNA vaccine is associated with its replication origin","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"In vitro and in vivo stability of plasmids in attenuated Salmonella enterica serovar typhimurium used as a carrier of DNA vaccine is associated with its replication origin","subitem_title_language":"en"},{"subitem_title":"DNAワクチンの運搬役としての弱毒化ネズミチフス菌においてプラスミドの安定性はその複製起点が関連する","subitem_title_language":"ja"}]},"item_type_id":"10001","owner":"5","path":["74","26"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2010-05-26"},"publish_date":"2010-05-26","publish_status":"0","recid":"769","relation_version_is_last":true,"title":["In vitro and in vivo stability of plasmids in attenuated Salmonella enterica serovar typhimurium used as a carrier of DNA vaccine is associated with its replication origin"],"weko_creator_id":"5","weko_shared_id":2},"updated":"2023-07-29T12:55:28.380266+00:00"}