{"created":"2023-05-15T09:57:00.511795+00:00","id":765,"links":{},"metadata":{"_buckets":{"deposit":"cde6ecc1-a2f7-4add-81b8-82b79a0f1ca1"},"_deposit":{"created_by":5,"id":"765","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"765"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00000765","sets":["74","74:26"]},"author_link":["4657","2692","1585","4658","4650","4654","4614","4740","2693","4651"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-12-22","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"156","bibliographicPageStart":"148","bibliographicVolumeNumber":"121","bibliographic_titles":[{"bibliographic_title":"Immunology letters","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Lectin isolated from the seeds of Momordica charantia (MCL) is a galactose-specific glycoprotein. To investigate the effects of MCL on cell activation, we analyzed the responses of BALB/c splenocytes, thymocytes, T cells and B cells on MCL stimulation. Proliferation assays showed that MCL selectively stimulates the B cell subset of splenocytes (p < 0.05) in a dose and time dependent manner and that this activation proceeds without the involvement of T cells. Flow cytometric analysis revealed that the fluorescein isothiocyanate (FITC)-labeled MCL binds to B cells, which was inhibited by specific sugars, including galactose. Mouse immunoglobulin (Ig) was able to inhibit MCL-induced proliferation of mouse B cells, suggesting MCL stimulates B cell activation via membrane Ig in the B cell surface. Moreover, after 96-h co-culture, MCL triggered splenocytes to produce a large amount of non-specific IgM in culture supernatants (p < 0.01). Additionally, MCL was shown to up-regulate the cell activation marker CD86, in a B cell subpopulation distinct from that affected by LPS. These data suggest that MCL is a T cell-independent B cell activator and a polyclonal Ig inducer, and provide further information on the immunomodulatory effect of MCL.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier 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Li","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4650","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Adachi, Takumi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4651","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimizu, Yuya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4614","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Goto, Yoshitaka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2693","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Toyama, Jun","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4654","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000106573887","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000106573887"}],"affiliationNames":[{"affiliationName":"宮崎大学","affiliationNameLang":"ja"},{"affiliationName":"University of Miyazaki","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"田中, 秀典","creatorNameLang":"ja"},{"creatorName":"タナカ, ヒデノリ","creatorNameLang":"ja-Kana"},{"creatorName":"Tanaka, 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良","creatorNameLang":"ja"},{"creatorName":"アカシ, リョウ","creatorNameLang":"ja-Kana"},{"creatorName":"Akashi, Ryo","creatorNameLang":"en"}],"familyNames":[{"familyName":"明石","familyNameLang":"ja"},{"familyName":"アカシ","familyNameLang":"ja-Kana"},{"familyName":"Akashi","familyNameLang":"en"}],"givenNames":[{"givenName":"良","givenNameLang":"ja"},{"givenName":"リョウ","givenNameLang":"ja-Kana"},{"givenName":"Ryo","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1585","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20253809","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20253809"}]},{"creatorNames":[{"creatorName":"Sawaguchi, Akira","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4657","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Iwata, Hiroyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4658","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Haga, Takeshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2692","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"2876.pdf","filesize":[{"value":"631.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"2876.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/765/files/2876.pdf"},"version_id":"04fd74cd-f2fe-48b2-b803-caabf7f6c3f6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Momordica charantia seed lectin, B cell activator, T cell independent, BALB/c mice","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characterization of lectin isolated from Momordica charantia seed as a B cell activator","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of lectin isolated from Momordica charantia seed as a B cell activator","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"5","path":["74","26"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2010-05-26"},"publish_date":"2010-05-26","publish_status":"0","recid":"765","relation_version_is_last":true,"title":["Characterization of lectin isolated from Momordica charantia seed as a B cell activator"],"weko_creator_id":"5","weko_shared_id":2},"updated":"2025-07-23T10:11:54.142537+00:00"}