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Establishing an efficient protoplast transient expression system for investigation of floral thermogenesis in aroids
http://hdl.handle.net/10458/00010394
http://hdl.handle.net/10458/000103943aaf473e-7fca-446d-af1f-1c1b60a59985
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Maekawa2022_Article_EstablishingAnEfficientProtopl (3.0 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2022-09-15 | |||||
| タイトル | ||||||
| タイトル | Establishing an efficient protoplast transient expression system for investigation of floral thermogenesis in aroids | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | Araceae | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | Symplocarpus renifolius | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | Alocasia odora | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | Mesophyll protoplast | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | Inflorescence | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | Protein localization | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
稲葉, 丈人
× 稲葉, 丈人× 稲葉, 靖子× Maekawa, Haruhiko× Otsubo, Miyabi× Sato, Mitsuhiko P.× 髙橋, 智子× Mizoguchi, Koichiro× Koyamatsu, Daiki× Inaba, Takehito |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Aroids contain many species with intense heat-producing abilities in their inflorescences. Several genes have been proposed to be involved in thermogenesis of these species, but biological tools for gene functional analyses are lacking. In this study, we aimed to develop a protoplast-based transient expression （PTE） system for the study of thermogenic aroids. Initially, we focused on skunk cabbage （Symplocarpus renifolius） because of its ability to produce intense as well as durable heat. In this plant, leaf protoplasts were isolated from potted and shoot tip-cultured plants with high efficiency （ca. 1.0 × 105/g fresh weight）, and more than half of these protoplasts were successfully transfected. Using this PTE system, we determined the protein localization of three mitochondrial energy-dissipating proteins, SrAOX, SrUCPA, and SrNDA1, fused to green fluorescent protein （GFP）. These three GFP-fused proteins were localized in MitoTracker-stained mitochondria in leaf protoplasts, although the green fluorescent particles in protoplasts expressing SrUCPA-GFP were significantly enlarged. Finally, to assess whether the PTE system established in the leaves of S. renifolius is applicable for floral tissues of thermogenic aroids, inflorescences of S. renifolius and another thermogenic aroid （Alocasia odora） were used. Although protoplasts were successfully isolated from several tissues of the inflorescences, PTE systems worked well only for the protoplasts isolated from the female parts （slightly thermogenic or nonthermogenic） of A. odora inflorescences. Our developed system has a potential to be widely used in inflorescences as well as leaves in thermogenic aroids and therefore may be a useful biological tool for investigating floral thermogenesis. | |||||
| 書誌情報 |
en : Plant Cell Reports 巻 41, 号 1, p. 263-275, 発行日 2021-10-26 |
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| 出版者 | ||||||
| 出版者 | Springer | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0721-7714 | |||||
| PubMed番号 | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 34704119 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1007/s00299-021-02806-1 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
