{"created":"2023-07-29T08:04:10.641337+00:00","links":{},"metadata":{"_buckets":{"deposit":"d2a1d9dd-2265-40ad-9711-76e51a845657"},"_deposit":{"created_by":5,"id":"6519.2","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"6519.2"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00006519.2","sets":["79","79:42"]},"author_link":["5752","33890","33891","1631","21835","33873","33874","33875","33876","33877","9842"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"615","bibliographicPageEnd":"62","bibliographicPageStart":"56","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"With the current worldwide pandemic of COVID-19, there is an urgent need to develop effective treatment and prevention methods against SARS-CoV-2 infection. We have previously reported that the proanthocyanidin （PAC） fraction in blueberry （BB） leaves has strong antiviral activity against hepatitis C virus （HCV） and human T-lymphocytic leukemia virus type 1 （HTLV-1）. In this study, we used Kunisato 35 Gou （K35） derived from the rabbit eye blueberry （Vaccinium virgatum Aiton）, which has a high PAC content in the leaves and stems. The mean of polymerization （mDP） of PAC in K35 was the highest of 7.88 in Fraction 8 （Fr8） from the stems and 12.28 of Fraction 7 （Fr7） in the leaves. The composition of BB-PAC in K35 is that most are B-type bonds with a small number of A-type bonds and cinchonain I as extension units. A strong antiviral effect was observed in Fr7, with a high polymerized PAC content in both the leaves and stems. Furthermore, when we examined the difference in the action of BB-PAC before and after SARS-CoV-2 infection, we found a stronger inhibitory effect in the pre-infection period. Moreover, BB-PAC Fr7 inhibited the activity of angiotensin II converting enzyme （ACE2）, although no effect was observed in a neutralization test of pseudotyped SARS-CoV-2. The viral chymotrypsin-like cysteine protease （3CLpro） of SARS-CoV-2 was also inhibited by BB-PAC Fr7 in leaves and stems. These results indicate that BB-PAC has at least two different inhibitory effects, and that it is effective in suppressing SARS-CoV-2 infection regardless of the time of infection.","subitem_description_type":"Abstract"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.bbrc.2022.04.072","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2022 The Authors. Published by Elsevier Inc."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006291X","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"菅本, 和寛"},{"creatorName":"スガモト, カズヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Sugamoto, Kazuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"5752","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"10274771","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10274771"}]},{"creatorNames":[{"creatorName":"齊藤, 暁"},{"creatorName":"サイトウ, アカツキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"33890","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"30621792","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=30621792"}]},{"creatorNames":[{"creatorName":"岡林, 環樹"},{"creatorName":"オカバヤシ, タマキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"33891","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"10359995","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10359995"}]},{"creatorNames":[{"creatorName":"國武, 久登"},{"creatorName":"クニタケ, ヒサト","creatorNameLang":"ja-Kana"},{"creatorName":"Kunitake, Hisato","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1631","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80289628","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80289628"}]},{"creatorNames":[{"creatorName":"森下, 和広"},{"creatorName":"モリシタ, カズヒロ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"21835","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80260321","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80260321"}]},{"creatorNames":[{"creatorName":"Tanaka, Yuri L.","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"33873","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Saito, Akatsuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"33874","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Goto, Yoh","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"33875","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakayama, Takayuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"33876","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okabayashi, Tamaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"33877","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Morishita, Kazuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"9842","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-06-23"}],"displaytype":"detail","filename":"1-s2.0-S0006291X22006027-main.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"1-s2.0-S0006291X22006027-main","url":"https://miyazaki-u.repo.nii.ac.jp/record/6519/files/1-s2.0-S0006291X22006027-main.pdf"},"version_id":"49769421-ab48-4475-822f-2053442d0b13"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"SARS-CoV-2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Roanthocyanidins","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Blueberry","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Angiotensin II converting Enzyme","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Viral chymotrypsin-like cysteine protease (3CLpro)","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Highly polymerized proanthocyanidins (PAC) components from blueberry leaf and stem significantly inhibit SARS-CoV-2 infection via inhibition of ACE2 and viral 3CLpro enzymes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Highly polymerized proanthocyanidins (PAC) components from blueberry leaf and stem significantly inhibit SARS-CoV-2 infection via inhibition of ACE2 and viral 3CLpro enzymes","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"5","path":["79","42"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-05-11"},"publish_date":"2022-05-11","publish_status":"0","recid":"6519.2","relation_version_is_last":true,"title":["Highly polymerized proanthocyanidins (PAC) components from blueberry leaf and stem significantly inhibit SARS-CoV-2 infection via inhibition of ACE2 and viral 3CLpro enzymes"],"weko_creator_id":"5","weko_shared_id":-1},"updated":"2023-07-29T08:04:13.506159+00:00"}