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Development of basophils in mongolian gerbils: Formation of basophilic cell clusters in the bone marrow after Nippostrongylus brasiliensis infection
http://hdl.handle.net/10458/1923
http://hdl.handle.net/10458/192354565296-9ce5-4c4e-abd2-c7072dae7b28
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
li76-1.pdf (1.5 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2009-03-02 | |||||
| タイトル | ||||||
| タイトル | Development of basophils in mongolian gerbils: Formation of basophilic cell clusters in the bone marrow after Nippostrongylus brasiliensis infection | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | basophil, mast cell, peroxidase, bone marrow, Monogolian gerbil, ultrastructure, Nippostrongylus brasiliensis | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Okada, Manabu
× Okada, Manabu× Nawa, Yukifumi× Horii, Yoichiro× Kitamura, Tadashi× Arizono, Naoki |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Development of basophilic leukocytes was studied in the Mongolian gerbil, Meriones unguiculatus, after infection with the nematode Nippostrongylus brasiliensis. After infection, peripheral blood basophilia developed and peaked at 2 weeks. In bone marrow sections, numbers of alcian blue+/safranine basophilic cells were increased. These cells did not bind berberine sulfate and were clearly distinguishable from the bone marrow- resident mast cells, safranine+ and berberine sulfate+. Alcian blue+/safranine cells were identified by electron microscopy as basophilic myelocytes in various stages of maturation. In the early period of infection, these cells had round-to-oval granules with a homogenous electron-dense matrix, a well-developed Golgi apparatus and rough endoplasmic reticulum, and a nonsegmented nucleus. By enzyme cytochemical analysis, intense peroxidase activity was demonstrated in all of the specific granules as well as in the rough endoplasmic reticulum and Golgi apparatus. Two weeks after infection, the number of bone marrow basophilic cells further increased, forming distinct clusters or islands composed of up to 100 cells each. On electron micrographs, the basophilic cells in these clusters appeared to be late- stage basophilic myelocytes, ie, having an increased number of granules, a less-conspicuous Golgi apparatus and rough endoplasmic reticulum, a horseshoe-shaped-to-lobulated nucleus, and reduced peroxidase activity. Eosinophils and mast cells were rarely found in the basophilic cell clusters. Four weeks after infection, the clusters had disappeared. These results show that gerbil basophilic myelocytes tend to form cell clusters in the bone marrow during their active proliferation. The comparative paucity of other cell lineages in basophilic cell clusters suggests that basophilia is generated from differentiation/proliferation of precommitted basophil progenitors independently from cells of other lineages. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Laboratory investigation : journal of technical methods and pathology 巻 76, 号 1, p. 89-97, 発行日 1997-01 |
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| 出版者 | ||||||
| 出版者 | Nature Publishing Group | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00236837 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 15300307 | |||||
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| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00713961 | |||||
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| 出版タイプ | AM | |||||
