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Sensitive analysis of arsenic species in urine by LC-MS/MS
http://hdl.handle.net/10458/6272
http://hdl.handle.net/10458/62728351c72b-0718-4be0-b85c-7e32e4adb206
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
本文 (249.5 kB)
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| Item type | その他 / Others(1) | |||||
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| 公開日 | 2020-06-21 | |||||
| タイトル | ||||||
| タイトル | Sensitive analysis of arsenic species in urine by LC-MS/MS | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Arsenic, Metabolite, LC-MS/MS, Omics, Pilot study | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_1843 | |||||
| 資源タイプ | other | |||||
| 著者 |
Yamauch, Takenori
× Yamauch, Takenori× Hata, Akihisa× Yamano, Yuko× Nakadate, Toshio |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Although it is familiar that arsenic is ingested routinely by food substance such as seaweed,species of arsenic metabolite in urine are not fully identified. In order to assess the toxicity of arsenic contained in food properly, it is necessary to identify unknown arsenic metabolites. One of the efficient approaches is omics approach with high-performance liquid chromatography-electrospray ionisation tandem mass spectrometry （HPLC-ESI-MS/MS） using urine both before and after arsenic ingestion. However, analysis on arsenic species by LC-MS/MS is much less common than that by high performance liquid chromatography with inductively coupled plasma mass spectrometry （HPLC-ICP-MS）. Thus, analysis on available arsenic standard （i.e. arsenobetine, monomethyl arsonous acid, dimethylarsinic acid, trimethylarsine oxide, arsenocholine and dimethylmonothioarsinic acid） by HPLC-ESI-MS/MS was conducted as a pilot study. All of them without monomethyl arsonous acid was detected in a picogram or femtogram order. Furthermore, most of them can be quantitied at least in a picogram order. However, a part of them such as dimethylmonothioarsinic acid was completely undetected or little detected when they were added to urine sample even in a ppm order. Thus, it indicated the presence of an inhibitor for ionization in urine, and also did the necessity of sample preparation before analysis. However, extremely high sensitivity of arsenic metabolites suggested the possibility of omics approach. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : 4th InternationalArsenic Symposium in MIYAZAKI 2015 p. 62-64, 発行日 2015-10 |
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| 出版者 | ||||||
| 出版者 | University of Miyazaki, IRISH | |||||
| 言語 | en | |||||
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| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
