@article{oai:miyazaki-u.repo.nii.ac.jp:00000551,
 author = {立石, 美加 and 堀井, 洋一郎 and Horii, Yoichiro and Maruyama, Haruhiko and 丸山, 治彦 and 名和, 行文 and Tsuchiya, Kimiyuki and 土屋, 公幸 and 牧村, 進 and Makimura, Susumu and Tateishi, Mika and Maruyama, Haruhiko and 丸山, 治彦 and Nawa, Yukifumi and Tsuchiya, Kimiyuki and 土屋, 公幸 and 牧村, 進 and Makimura, Susumu},
 issue = {6},
 journal = {The journal of veterinary medical science},
 month = {Jun},
 note = {ミラルディア（Millardia meltada）のIgG抗体をイムノアフィニティークロマトグラフィー法で精製し, ウサギ抗血清を作成した. この抗体を用いて, 寄生虫特異的ミラルデイア抗体をELISAにて測定したところ, 従来の方法に比べ著しい感度の上昇が認められた. ミラルデイアはStrongyloides venezuelensisとNippostrongylus brasiliensis感染に対して効率的な抗体産生を行っていることから, この動物の寄生虫に対する高感受性は一般的な免疫不全によるものではないと考えられる.
\nImmunoglobulin G （IgG） of Indian soft-furred rat, Millardia meltada, was purified by an immunoaffinity chromatography and antibodies against it was raised in rabbit. Using this rabbit anti-M. meltada IgG antibody, sensitivity of enzyme-linked immunosorbent assay （ELISA） to measure parasite-specific antibodies in the sera of M. meltada was markedly enhanced than the previous method using rabbit anti-mouse IgCi and rabbit anti-rat IgG antibodies, which could cross-react to M. meltada IgG. Since M. meltada could effectively produce circulating antibodies against two intestinal helminths, Strongyloides venezuelensis and Nippostrongylus brasiliensis, the high susceptibility of this animal to an array of parasites seems to be not due to general immunological deficiency.},
 pages = {491--494},
 title = {Sensitive Enzyme-Linked Immunosorbent Assay (ELISA) to Measure Parasite-Specific Antibodies of Indian Soft-Furred Rats, Millardia meltada},
 volume = {59},
 year = {1997},
 yomi = {タテイシ, ミカ and ホリイ, ヨウイチロウ and マルヤマ, ハルヒコ and ナワ, ユキフミ and ツチヤ, キミユキ and マキムラ, ススム and マルヤマ, ハルヒコ and ツチヤ, キミユキ and マキムラ, ススム}
}