{"created":"2023-05-15T09:56:50.033391+00:00","id":538,"links":{},"metadata":{"_buckets":{"deposit":"6f69aaa9-95e8-472c-a86e-783b666e78d8"},"_deposit":{"created_by":5,"id":"538","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"538"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00000538","sets":["74","74:26"]},"author_link":["2104","3134","3135","932"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"712","bibliographicPageStart":"706","bibliographicVolumeNumber":"215","bibliographic_titles":[{"bibliographic_title":"Biochemical and biophysical research communications","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Phosphorylation of demembranated fowl sperm proteins during incubation with [γ-32P]ATP and various protein kinase substrate peptides at 30°C was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）. A marked difference in phosphorylation was observed in a 30 kDa protein. This protein was strongly phosphorylated after the addition of Kemptide, a cAMP-dependent protein kinase （PKA） substrate peptide; Syntide 2, a calmodulin-dependent protein kinase II substrate peptide; a protein kinase C （PKC） substrate peptide; as well as control samples but only slightly phosphorylated in the presence of a myosin light chain kinase （MLCK） substrate peptide. The motility of demembranated spermatozoa at 30°C remained high in control samples and following the addition of Kemptide, Syntide 2 and PKC substrate peptide, but decreased markedly following the addition of MLCK substrate peptide. These results suggest that the 30 kDa protein is identified as a substrate for MLCK or a MLCK-like protein in fowl spermatozoa and that phosphorylation-dephosphorylation of this protein is involved in the regulation of flagellar movement at 30°C.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00564395","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006291X","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Ashizawa, Koji","creatorNameLang":"en"},{"creatorName":"芦沢, 幸二","creatorNameLang":"ja"},{"creatorName":"アシザワ, コウジ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Ashizawa","familyNameLang":"en"},{"familyName":"芦沢","familyNameLang":"ja"},{"familyName":"アシザワ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Koji","givenNameLang":"en"},{"givenName":"幸二","givenNameLang":"ja"},{"givenName":"コウジ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"932","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wishart, Graham J.","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3134","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hashimoto, Kei","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3135","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000106573887","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000106573887"}],"affiliationNames":[{"affiliationName":"宮崎大学","affiliationNameLang":"ja"},{"affiliationName":"University of Miyazaki","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"續木, 靖浩","creatorNameLang":"ja"},{"creatorName":"ツヅキ, ヤスヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Tsuzuki, Yasuhiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"續木","familyNameLang":"ja"},{"familyName":"ツヅキ","familyNameLang":"ja-Kana"},{"familyName":"Tsuzuki","familyNameLang":"en"}],"givenNames":[{"givenName":"靖浩","givenNameLang":"ja"},{"givenName":"ヤスヒロ","givenNameLang":"ja-Kana"},{"givenName":"Yasuhiro","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2104","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00236928","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00236928"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"ashizawa56.pdf","filesize":[{"value":"788.4 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