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Abnormal GABA neurotransmission in a Kcnq2 model of early-onset epilepsy
http://hdl.handle.net/10458/6378
http://hdl.handle.net/10458/6378d784e92e-8b6d-4784-ba74-607c3a8e6d98
| 名前 / ファイル | ライセンス | アクション |
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本文 (2.2 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2020-06-21 | |||||
| タイトル | ||||||
| タイトル | Abnormal GABA neurotransmission in a Kcnq2 model of early-onset epilepsy | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | KCNQ2, GABA, interneuron, neonatal epilepsy, knock-in | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
内田, 琢
× 内田, 琢× Uchida, Taku× Lossin, Christoph× Ihara, Yukiko× Deshimaru, Masanobu× Yanagawa, Yuchio× Koyama, Susumu× Hirose, Shinichi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Objective: Mutations of the KCNQ2 gene, which encodes the Kv7.2 subunit of voltagegated M-type potassium channels, have been associated with epilepsy in the neonatal period. This developmental stage is unique in that the neurotransmitter gamma aminobutyric acid （GABA）, which is inhibitory in adults, triggers excitatory action due to a reversed chloride gradient. Methods: To examine whether KCNQ2-related neuronal hyperexcitability involves neonatally excitatory GABA, we examined 1-week-old knockin mice expressing the Kv7.2 variant p.Tyr284Cys （Y284C）. Results: Brain slice electrophysiology revealed elevated CA1 hippocampal GABAergic interneuron activity with respect to presynaptic firing and postsynaptic current frequency. Blockade with the GABAA receptor antagonist bicuculline decreased ictal-like bursting in brain slices with lowered divalent ion concentration, which is consistent with GABA mediating an excitatory function that contributes to the hyperexcitability observed in mutant animals. Significance: We conclude that excitatory GABA contributes to the phenotype in these animals, which raises the question of whether this special type of neurotransmission has broader importance in neonatal epilepsy than is currently recognized. |
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| 書誌情報 |
Epilepsia 巻 58, 号 8, p. 1430-1439, 発行日 2015-04 |
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| 出版者 | Wiley | |||||
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| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00139580 | |||||
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| 出版タイプ | AM | |||||
