{"created":"2023-05-15T09:56:49.262455+00:00","id":520,"links":{},"metadata":{"_buckets":{"deposit":"cf43d3e3-79ee-42f2-b2ac-e95d6a93e749"},"_deposit":{"created_by":5,"id":"520","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"520"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00000520","sets":["74","74:26"]},"author_link":["21426","25096","2632","25106"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"404","bibliographicPageStart":"398","bibliographicVolumeNumber":"106","bibliographic_titles":[{"bibliographic_title":"Journal of bioscience and bioengineering","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"An extracellular protein exhibiting β-xylosidase activity was purified from the culture filtrate of a filamentous fungus, Aspergillus japonicus strain MU-2, grown on oat spelt xylan. The purified enzyme was a monomeric glycoprotein with an apparent Mr of 113.2 kDa as estimated by SDS-PAGE. β-Xylosidase activity was optimal at pH 4.0 and 70°C. The enzyme also showed β-glucosidase and α-l-arabinofuranosidase activities. The genomic DNA and cDNA encoding this protein were cloned and sequenced. Southern blot analysis indicated that the β-xylosidase gene （xylA） was present as a single copy in the genome. An open reading frame, consisting of 2412 bp, was not interrupted by introns, and it encoded a presumed signal peptide of 17 amino acids and a mature protein of 787 amino acids. The deduced amino acid sequence of the xylA gene product showed a high degree of identity （69%） to the primary structure of the Aspergillus niger β-xylosidase XlnD that belongs to the glycoside hydrolase family 3. Moreover, the xylA gene was functionally expressed in the yeast Pichia pastoris.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本生物工学会","subitem_publisher_language":"ja"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11307678","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13891723","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"脇山, 元気","creatorNameLang":"ja"},{"creatorName":"ワキヤマ, モトキ","creatorNameLang":"ja-Kana"},{"creatorName":"Wakiyama, Motoki","creatorNameLang":"en"}],"familyNames":[{"familyName":"脇山","familyNameLang":"ja"},{"familyName":"ワキヤマ","familyNameLang":"ja-Kana"},{"familyName":"Wakiyama","familyNameLang":"en"}],"givenNames":[{"givenName":"元気","givenNameLang":"ja"},{"givenName":"モトキ","givenNameLang":"ja-Kana"},{"givenName":"Motoki","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"21426","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"吉原, 浩司","creatorNameLang":"ja"},{"creatorName":"ヨシハラ, コウジ","creatorNameLang":"ja-Kana"},{"creatorName":"Yoshihara, Koji","creatorNameLang":"en"}],"familyNames":[{"familyName":"吉原","familyNameLang":"ja"},{"familyName":"ヨシハラ","familyNameLang":"ja-Kana"},{"familyName":"Yoshihara","familyNameLang":"en"}],"givenNames":[{"givenName":"浩司","givenNameLang":"ja"},{"givenName":"コウジ","givenNameLang":"ja-Kana"},{"givenName":"Koji","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"25096","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"林, 幸男","creatorNameLang":"ja"},{"creatorName":"ハヤシ, サチオ","creatorNameLang":"ja-Kana"},{"creatorName":"Hayashi, Sachio","creatorNameLang":"en"}],"familyNames":[{"familyName":"林","familyNameLang":"ja"},{"familyName":"ハヤシ","familyNameLang":"ja-Kana"},{"familyName":"Hayashi","familyNameLang":"en"}],"givenNames":[{"givenName":"幸男","givenNameLang":"ja"},{"givenName":"サチオ","givenNameLang":"ja-Kana"},{"givenName":"Sachio","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"25106","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"太田, 一良","creatorNameLang":"ja"},{"creatorName":"オオタ, カズヨシ","creatorNameLang":"ja-Kana"},{"creatorName":"Ohta, Kazuyoshi","creatorNameLang":"en"}],"familyNames":[{"familyName":"太田","familyNameLang":"ja"},{"familyName":"オオタ","familyNameLang":"ja-Kana"},{"familyName":"Ohta","familyNameLang":"en"}],"givenNames":[{"givenName":"一良","givenNameLang":"ja"},{"givenName":"カズヨシ","givenNameLang":"ja-Kana"},{"givenName":"Kazuyoshi","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2632","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"jbb106398.pdf","filesize":[{"value":"2.2 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β-Xylosidase from Aspergillus japonicus and Sequence Analysis of the Encoding Gene","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"5","path":["74","26"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2009-02-03"},"publish_date":"2009-02-03","publish_status":"0","recid":"520","relation_version_is_last":true,"title":["Purification and Properties of an Extracellular β-Xylosidase from Aspergillus japonicus and Sequence Analysis of the Encoding Gene"],"weko_creator_id":"5","weko_shared_id":-1},"updated":"2024-12-03T06:50:18.132629+00:00"}