@article{oai:miyazaki-u.repo.nii.ac.jp:00000518, author = {八幡, 昌紀 and Yahata, Masaki and 黒木, 宏憲 and 國武, 久登 and Kunitake, Hisato and 長野, 克也 and Nagano, Katsuya and 藪谷, 勤 and Yabuya, Tsutomu and 山下, 研介 and 小松, 春喜 and Komatsu, Haruki and 八幡, 昌紀 and Yahata, Masaki and Kurogi, Hironori and 長野, 克也 and Nagano, Katsuya and 藪谷, 勤 and Yabuya, Tsutomu and Yamashita, Kensuke and 小松, 春喜 and Komatsu, Haruki}, issue = {4}, journal = {園藝學會雜誌}, month = {Jul}, note = {晩白柚'の実生から得られた半数体における雌性および雄性配偶子の生殖機能を評価するために, 半数体と種々の二倍体カンキツ品種との交雑を行った.半数体に二倍体の花粉を授粉した場合では全く着果しなかったが, 半数体を花粉親とした場合, 4つの交雑組合せにおいて完全種子が得られた.これらの完全種子は正常に発芽し, 多くの二倍体実生が得られた.これらの実生は旺盛に成長し, '晩白柚'の形態的特徴である翼葉を有していた.さらに, '清見'と半数体との交雑から得られた1個体の実生について, RAPD分析およびCMA染色による染色体構成を解析したところ, 雑種であることが確認された.これらの結果から, 本半数体では正常な花粉(n=9)が形成されていることが示唆された. \nTo evaluate the reproductive potential of female and male gametes in a haploid plant derived from seedlings of 'Banpeiyu' pummelo, we performed crosses between the haploid and several diploid citrus cultivars. In the crosses with the diploid cultivars as pollen parents, no fruit set on the haploid in all cross combinations. However, developed seeds were obtained in four cross combinations, when some monoembryonic diploid cultivars were pollinated with pollen of the haploid. These seeds germinated normally and developed into diploid, vigorous seedlings with large wing leaves, typical of 'Banpeiyu' pummelo. Furthermore, the hybridity of a seedling obtained from the cross between 'Kiyomi' tangor and the haploid was confirmed by random amplified polymorphic DNA (RAPD) analysis and chromosome composition by chromomycin A_3 (CMA) staining. These results suggest that the haploid produced fertile pollen grains (n=9).}, pages = {281--288}, title = {Evaluation of Reproductive Functions in a Haploid Pummelo by Crossing with Several Diploid Citrus Cultivars}, volume = {74}, year = {2005}, yomi = {ヤハタ, マサキ and クロギ, ヒロノリ and クニタケ, ヒサト and ナガノ, カツヤ and ヤブヤ, ツトム and ヤマシタ, ケンスケ and コマツ, ハルキ and ヤハタ, マサキ and ナガノ, カツヤ and ヤブヤ, ツトム and コマツ, ハルキ} }