{"created":"2023-05-15T10:00:46.361253+00:00","id":5170,"links":{},"metadata":{"_buckets":{"deposit":"5442c1f5-2848-4cf1-ae44-c84659760cb0"},"_deposit":{"created_by":5,"id":"5170","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"5170"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00005170","sets":["76","76:64"]},"author_link":["3231","21732","27920","27924","27919","1667"],"control_number":"5170","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-07-19","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageStart":"e12301","bibliographicVolumeNumber":"37","bibliographic_titles":[{"bibliographic_title":"Journal of Food Safety","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Ascarid larva migrans syndrome （ascarid LMS） is caused by ascarid roundworms including Ascaris suum. In East Asia, ascarid LMS has been considered as a food-borne disease in adulthood who has the dietary habitat of consuming raw or lightly cooked meat and organ meats. To evaluate the potential risk of A. suum infection from these foods, Ascaris specific real-time PCR was developed. The assay could constantly detect A. suum DNA up to 10 fg. Its specificity was confirmed by non-amplification with Toxocara canis and Toxocara cati DNA. A. suum DNA could be amplified from not only a single larva but also mouse liver spiked with a larva. Moreover, the assay could detect A. suum DNA in experimentally infected mouse liver, and showed higher sensitivity than a conventional digestion method. This real-time PCR assay would be useful for detecting the A. suum larval contamination in meat and organ meats.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Citation:\nNguyen YTH, Wang Z, Maruyama H, Horii Y, Nonaka N, Yoshida A. Evaluation of real-time PCR assay for the detection of Ascaris suum contamination in meat and organ meats. J Food Saf. 2017; 37:e12301. https://doi.org/10.1111/jfs.12301","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Wiley","subitem_publisher_language":"en"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1111/jfs.12301","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2016 Wiley Periodicals, Inc.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"17454565","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Yen, Thi Hoang  Nguyen","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yen","familyNameLang":"en"}],"givenNames":[{"givenName":"Thi Hoang  Nguyen","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"27919","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Zhenzhen, Wang","creatorNameLang":"en"}],"familyNames":[{"familyName":"Zhenzhen","familyNameLang":"en"}],"givenNames":[{"givenName":"Wang","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"27920","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000106573887","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000106573887"}],"affiliationNames":[{"affiliationName":"宮崎大学","affiliationNameLang":"ja"},{"affiliationName":"University of Miyazaki","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"丸山, 治彦","creatorNameLang":"ja"},{"creatorName":"マルヤマ, ハルヒコ","creatorNameLang":"ja-Kana"},{"creatorName":"Maruyama, Haruhiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"丸山","familyNameLang":"ja"},{"familyName":"マルヤマ","familyNameLang":"ja-Kana"},{"familyName":"Maruyama","familyNameLang":"en"}],"givenNames":[{"givenName":"治彦","givenNameLang":"ja"},{"givenName":"ハルヒコ","givenNameLang":"ja-Kana"},{"givenName":"Haruhiko","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3231","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90229625","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90229625"}]},{"creatorNames":[{"creatorName":"Horii, Yoichiro","creatorNameLang":"en"},{"creatorName":"堀井, 洋一郎","creatorNameLang":"ja"},{"creatorName":"ホリイ, ヨウイチロウ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Horii","familyNameLang":"en"},{"familyName":"堀井","familyNameLang":"ja"},{"familyName":"ホリイ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Yoichiro","givenNameLang":"en"},{"givenName":"洋一郎","givenNameLang":"ja"},{"givenName":"ヨウイチロウ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"1667","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"野中, 成晃","creatorNameLang":"ja"},{"creatorName":"ノナカ, ナリアキ","creatorNameLang":"ja-Kana"},{"creatorName":"Nonaka, Nariaki","creatorNameLang":"en"}],"familyNames":[{"familyName":"野中","familyNameLang":"ja"},{"familyName":"ノナカ","familyNameLang":"ja-Kana"},{"familyName":"Nonaka","familyNameLang":"en"}],"givenNames":[{"givenName":"成晃","givenNameLang":"ja"},{"givenName":"ナリアキ","givenNameLang":"ja-Kana"},{"givenName":"Nariaki","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"21732","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000106573887","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000106573887"}],"affiliationNames":[{"affiliationName":"宮崎大学","affiliationNameLang":"ja"},{"affiliationName":"University of Miyazaki","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"吉田, 彩子","creatorNameLang":"ja"},{"creatorName":"ヨシダ, アヤコ","creatorNameLang":"ja-Kana"},{"creatorName":"Yoshida, Ayako","creatorNameLang":"en"}],"familyNames":[{"familyName":"吉田","familyNameLang":"ja"},{"familyName":"ヨシダ","familyNameLang":"ja-Kana"},{"familyName":"Yoshida","familyNameLang":"en"}],"givenNames":[{"givenName":"彩子","givenNameLang":"ja"},{"givenName":"アヤコ","givenNameLang":"ja-Kana"},{"givenName":"Ayako","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"27924","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20343486","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20343486"}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"food-borne disease","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"parasite","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"real-time PCR","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"meat","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"organ meats","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Evaluation of real-time PCR assay for the detection of Ascaris suum contamination in meat and organ meats","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Evaluation of real-time PCR assay for the detection of Ascaris suum contamination in meat and organ meats","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"5","path":["76","64"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-06-21"},"publish_date":"2020-06-21","publish_status":"0","recid":"5170","relation_version_is_last":true,"title":["Evaluation of real-time PCR assay for the detection of Ascaris suum contamination in meat and organ meats"],"weko_creator_id":"5","weko_shared_id":-1},"updated":"2025-01-15T04:58:34.810648+00:00"}