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Evaluation of real-time PCR assay for the detection of Ascaris suum contamination in meat and organ meats
http://hdl.handle.net/10458/6020
http://hdl.handle.net/10458/6020a287723f-48b3-40f0-a152-02341c97334f
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-06-21 | |||||
| タイトル | ||||||
| タイトル | Evaluation of real-time PCR assay for the detection of Ascaris suum contamination in meat and organ meats | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | food-borne disease | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | parasite | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | real-time PCR | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | meat | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | organ meats | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| 著者 |
Yen, Thi Hoang Nguyen
× Yen, Thi Hoang Nguyen× Zhenzhen, Wang× 丸山, 治彦× 堀井, 洋一郎× 野中, 成晃× 吉田, 彩子 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Ascarid larva migrans syndrome （ascarid LMS） is caused by ascarid roundworms including Ascaris suum. In East Asia, ascarid LMS has been considered as a food-borne disease in adulthood who has the dietary habitat of consuming raw or lightly cooked meat and organ meats. To evaluate the potential risk of A. suum infection from these foods, Ascaris specific real-time PCR was developed. The assay could constantly detect A. suum DNA up to 10 fg. Its specificity was confirmed by non-amplification with Toxocara canis and Toxocara cati DNA. A. suum DNA could be amplified from not only a single larva but also mouse liver spiked with a larva. Moreover, the assay could detect A. suum DNA in experimentally infected mouse liver, and showed higher sensitivity than a conventional digestion method. This real-time PCR assay would be useful for detecting the A. suum larval contamination in meat and organ meats. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Citation: Nguyen YTH, Wang Z, Maruyama H, Horii Y, Nonaka N, Yoshida A. Evaluation of real-time PCR assay for the detection of Ascaris suum contamination in meat and organ meats. J Food Saf. 2017; 37:e12301. https://doi.org/10.1111/jfs.12301 |
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| 言語 | en | |||||
| 書誌情報 |
en : Journal of Food Safety 巻 37, 号 2, p. e12301, 発行日 2016-07-19 |
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| 出版者 | ||||||
| 出版者 | Wiley | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 17454565 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1111/jfs.12301 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | © 2016 Wiley Periodicals, Inc. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
